Producción CyT
Libro de resúmenes - CHEMICAL ANALYSIS, BIOLOGICAL IN VITRO AND IN VIVO PROPERTIES AND BEHAVIORAL EFECTS OF Oxalis erythrorhiza GILLIES EX HOOKER ET ARNOTT (OXALIDACEAE)
Congreso
Autoría:
Gómez, Jessica ; REY, MARIANA ; Kruse, M.S ; Gamarra-Luques, C. ; Zaragoza J ; Lima, B ; Simirgiotis, M J. ; Coirini, H. ; Tapia, A.Fecha:
2024Editorial y Lugar de Edición:
Sociedad de Biología de CuyoResumen *
Oxalis erythrorhiza Gillies ex Hooker et Arnott (Oxalidaceae), native to the central Andean region of Argentina, is consumed to treat health disorders, despite the lack of scientific support. In this work, a decoction (10%W/V, DOe) and a methanolic global extract (MGEOe) from its aerial parts were studied. The metabolite profiling was determined with ultrahigh resolution liquid chromatography orbitrap MS analysis (UHPLC-ESI-OT-MS) and ultra-high-resolution liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (UHPLC-ESI-QTOF-MS), revealing thirty-eight compounds that includes flavone, isoflavones, fatty acids, terpenes, phenolic acids, and sterols. The antioxidant properties were assessed through 2,2-diphenyl-1-picrylhydrazyl (DPPH), trolox equivalent antioxidant activity (TEAC), ferric-reducing antioxidant power (FRAP), and inhibition of lipid peroxidation in erythrocytes (ILP) assays, demonstrating strong antioxidant activities. The cytotoxic effects were tested in HCT-116 tumoral cell line, showing selective actions. DOe was evaluated in an animal model of insulin-resistance. Male SD rats received sucrose (10%W/V, S), a half dilution of DOe (5%W/V) with sucrose (HDOeS) or DOe with sucrose (DOeS) from PND21 to PND61. After this, the anxiety-like behavior and spatial memory were assessed using the open field (OF) and the novel object location (NOL) tests, respectively. Serum parameters glycemia (G), total cholesterol (C) and tryglicerides (T) were measured using commercial kits. Only HDOeS exhibited lower anxiety-like behavior in OF and improved performance in NOL compared to S. Furthermore, DOeS showed reduced serum parameters, while HDOeS presented lower C levels than S. The beneficial properties of these preparations could be attributed to the identified metabolites. Although further studies are needed to elucidate the precise mechanisms underlying these actions, the present results highlight O. erythrorhiza as a promising source of compounds applicable in health. (PICT2019-623). Información suministrada por el agente en SIGEVAPalabras Clave
TEACOXALIS ERYTHRORHIZAFRAPPHYTOMEDICINEDPPHBEHAVIORAL TESTSUHPLC-ESI-OT-MSILP