Producción CyT
IGCC - Antigiardial activity of palm extracts from the colombian amazon: mechanisms of cell death
Congreso
Autoría:
Gracia Bustos, Juan ; Luna Pizarro Gabriel ; Patolsky, Rocío ; Joray, María Belén ; Villalba Viscaino ; Galeano, P ; Espitia Almeida ; Correa Munera ; Ozturk M ; ROPOLO, ANDREA SILVANA ; Feliziani, Constanza ; Touz, María Carolina ; Laiolo, JerónimoFecha:
2025Editorial y Lugar de Edición:
IGCCResumen *
Giardiasis is a common parasitic infection caused by the protozoan Giardia lamblia, leading to gastrointestinal disturbances such as diarrhea, abdominal pain, and bloating. It is one of the most prevalent waterborne diseases worldwide, particularly affecting children in developed and developing countries. Despite the availability of treatments such as metronidazole, the increasing resistance of G. lamblia to conventional drugs and the side effects associated with long-term use have led to a search for alternative therapies. In this context, the therapeutic potential of plants from the Colombian Amazon has garnered significant interest due to their long history of use in folk medicine for treating various gastrointestinal disorders. This study evaluates the giardicidal activity of 15 crude hydroethanolic extracts from Amazonian plants against G. lamblia trophozoites. Using the MTT assay, the effectiveness of these extracts was tested at a concentration of 500 µg/mL against Giardia trophozoites (genotype A, WB/1267). Among the tested extracts, Attalea butyracea fruit extract (P-2) exhibited the strongest giardicidal activity, with a half-maximal inhibitory concentration (IC50) of 62.10 ± 6.57 µg/mL against the WB/1267 strain, and 100.90 ± 3.40 µg/mL against the GS/M strain (genotype B). Further analysis of P-2 revealed that at its IC50 concentration, it induced early apoptosis, while a significant increase in late apoptosis and necrosis was observed at 2xIC50. Immunofluorescence assay (IFA) and confocal microscopy confirmed chromatin condensation in treated trophozoites, and flow cytometry showed G1/S phase cell cycle arrest. Additionally, oxidative stress was induced, marked by increased reactive oxygen species (ROS), and structural damage was observed via IFA and transmission electron microscopy. Notably, Attalea butyracea extract synergized with metronidazole, the standard treatment for giardiasis. These findings highlight the potential of Colombian Amazon palm extracts, particularly Attalea butyracea, as promising candidates for further therapeutic development in the treatment of giardiasis. Información suministrada por el agente en SIGEVAPalabras Clave
COLOMBIAN AMAZONPLANT EXTRACTSMEDICINAL PLANTSGIARDIA LAMBLIAGIARDICIDAL ACTIVITY