Producción CyT

Revista Medicina - EXPLORING NEUROPROTECTIVE EFFECTS OF STEVIOSIDE, A GLYCOSIDE DERIVED FROM STEVIA REBAUDIANA: IN VITRO AND IN VIVO STUDIES

Congreso

Autoría:

PASTORE, VALENTINA ; Colettis, Natalia ; De Tezano Pinto, F. ; Marco, A. ; Carolina Marcucci ; Marina Rademacher ; Mariel Marder.

Fecha:

2023

Editorial y Lugar de Edición:

Fundación Revista Medicina

ISSN:

1669-9106

Resumen *

Epilepsy is a chronic neurological disorder characterized by recurring seizures, with neuronal hyperexcitability and oxidative damage from free radicals being key factors in its development. Current antiepileptic drugs control seizures in 70% of cases, while 30% are treatment-resistant. In Latin America and the Caribbean, 68.2% of countries use natural resources for seizures. Medicinal natural products and structural modification of active compounds are sought for high-impact public health disorders. Several preclinical and clinical studies suggest the use of stevia (Stevia rebaudiana B.) and its derivatives with therapeutic and pharmacological applications, as they exhibit a variety of biological activities. Here, we evaluate the neuroprotective activity of a stevia derivative, stevioside (STV). We worked in vitro with human neuroblastoma cells (SH-SY5Y), which were treated under 4 conditions: A) STV (1-100 µM, 24 h); B) PTZ, a cytotoxic convulsant compound (20 mM, 24 h); C) H2O2 (1 mM, 48 h); D) STV (30 and 100 µM, 24 h) plus B or C. It was observed that STV is not cytotoxic up to 100 µM. Furthermore, pretreatment of cells with STV prior to PTZ and H2O2 treatments reversed both the damage caused by PTZ and oxidative damage from H2O2, suggesting that STV is a promising agent capable of preventing oxidative stress inherent to seizure episodes. On the other hand, in in vivo assays in male Swiss mice, following National Institute of Health (NIH) protocols, STV at 100 mg/kg, i.p., provided 75% protection of mice treated with PTZ (85 mg/kg, s.c.) at 4 hours after administration. Additionally, mice brains were homogenated and antioxidant assays were performed. STV showed a decrease in TBARs formation (P<0,0001) and an increase in endogenous antioxidant agents, GSH (P<0,01), compared to the PTZ group. Therefore, STV appears to be a potential anticonvulsant agent whose mechanism of action could be associated with the inhibition of reactive oxygen species production. Información suministrada por el agente en SIGEVA

Palabras Clave

STEVIA REBAUDIANA BEPILEPSYOXIDATIVE DAMAGENEUROPROTECTIVE