Producción CyT
Capítulo de Libro
Autoría
Chan, Francis Ka Ming
;
Moriwaki, Kenta
;
DE ROSA, MARIA JOSE
Fecha
2013
Editorial y Lugar de Edición
Humana Press
Libro
Immune Homeostasis-Methods and Protocols
(pp. 65-70)
Humana Press
Humana Press
ISBN
978-1-62703-289-6
Resumen
Información suministrada por el agente en
SIGEVA
Apoptosis and necrosis are two major forms of cell death observed in normal and disease pathologies. Although there are many assays for detection of apoptosis, relatively few assays are available for measuring necrosis. A key signature for necrotic cells is the permeabilization of the plasma membrane. This event can be quantified in tissue culture settings by measuring the release of the intracellular enzyme lactate dehydrogenase (LDH). When combined with other methods, measuring LDH release is...
Apoptosis and necrosis are two major forms of cell death observed in normal and disease pathologies. Although there are many assays for detection of apoptosis, relatively few assays are available for measuring necrosis. A key signature for necrotic cells is the permeabilization of the plasma membrane. This event can be quantified in tissue culture settings by measuring the release of the intracellular enzyme lactate dehydrogenase (LDH). When combined with other methods, measuring LDH release is a useful method for the detection of necrosis. In this chapter, we describe the step-by-step procedure for detection of LDH release from necrotic cells using a microtiter plate-based colorimetric absorbance assay.
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Palabras Clave
PROGRAMMED NECROSISLACTATE DEHYDROGENASEMEMBRANE LEAKAGENECROPTOSIS
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