Producción CyT
Trypanosoma cruzi Virulence Factors for the Diagnosis of Chagas’ Disease
Articulo
Autoría:
CHAIN, CECILIA YAMIL ; Pires Souto, Dênio Emanuel ; SBARAGLINI, MARIA LAURA ; Labriola, Carlos Alberto ; DAZA MILLONE, MARIA ANTONIETA ; RAMIREZ, EDUARDO ALEJANDRO ; CISNEROS, JOSÉ SEBASTIÁN ; Lopez Albizu, Maria Constanza ; Scollo, Karenina ; Kubota, Lauro T. ; Ruiz, Andrés Mariano ; Vela, Maria ElenaFecha:
2019Editorial y Lugar de Edición:
American Chemical SocietyRevista:
ACS Infectious Diseases, vol. 5 (pp. 1813-1819) - ISSN 2373-8227American Chemical Society
ISSN:
2373-8227Resumen
trans-Sialidase and cruzipain are important virulence factors from Trypanosoma cruzi, the etiological agent of Chagas disease, that have highly antigenic domains in their structure and were reported as potential tools for diagnosis of the illness. The aim of the present study is to assess the possibility of using cruzipain and the catalytic domain of trans-sialidase in a Surface Plasmon Resonance-based immunosensor for the diagnosis of chronic Chagas disease. Immunoassays carried out with canine sera verified that cruzipain allows the detection of anti-Trypanosoma cruzi antibodies whereas recombinant trans-sialidase did not yield specific detections, due to the high dilutions of serum used in the immunoassays that hinder the possibility to sense the specific low titer antibodies. The developed cruzipain-based biosensor, whose price per assay is comparable to a commercial enzyme-linked immunosorbent assay (ELISA), was successfully applied for the rapid quantification of specific antibodies against Trypanosoma cruzi in fresh human sera showing an excellent agreement with ELISAPalabras Clave
SURFACE PLASMON RESONANCEDIAGNOSTICSCRUZIPAINTRYPANOSOMA CRUZICHAGAS'DISEASETRANS-SIALIDASE