Artemia salina as a bioindicator for heavy metals
Artículo
Fecha:
2024Editorial y Lugar de Edición:
Producciones académicas de PosgradoRevista:
Divulgatio, vol. 8 - ISSN 2591-3530Producciones académicas de Posgrado
ISSN:
2591-3530Resumen *
Within our country, water toxicity assays are predominantly carried out usingphysicochemical analyses. While these parameters are accepted by current localregulations, additional regulations are being implemented around the globe, one of which isknown as ´biomonitoring.´ This involves evaluating toxicity in living organisms to study thepotential effects of samples on them. This includes considering the possibility of synergisticeffects of compounds present in the water, although the absence of synergy or even thepresence of antagonistic effects can also occur. Toxicity resulting from environmentalcontamination cannot solely be explained through the analysis of individual parameters.Thus, the only way to evaluate its toxic potency is through the application of toxicity assaysor tests using standardized living organisms for this purpose. In this study, Artemia salina, aspecies of crustacean found in brackish waters, was used. The mobility and viability of theArtemia were evaluated using an infrared microbeam motion counter and microscopiccounting, respectively. The Artemia were incubated with different metals for for 0, 1, and 24hours. Effects produced by Cu2+, Zn2+, Pb2+ at concentrations ranging from 0 to 300 mg/Lwere examined. Additionally, morphologies were analyzed only at 24 hours of exposure toeach metal to assess macroscopic toxic effects. No significant changes were recorded until24 hours, a concentration-dependent increase in mortality was observed for all tested metals.From these data, the lethal concentration 50 (LC50) was determined for the different metals,with Pb+2 being the most toxic at an LC50 of 13.40 mg/L, followed by Zn2+ (45.02 mg/L) andCu2+ (149.63 mg/L). A decrease in movement was observed at 24 hours across all testedconcentrations. Finally, morphological changes were observed primarily in thegastrointestinal tract, potentially resulting from bioaccumulation. In conclusion, using Artemiasalina as a bioindicator would enable the assessment of compound toxicity in solution overa short period, in a simple and economically accessible manner. Información suministrada por el agente en SIGEVAPalabras Clave
toxicityheavy metalsbioindicator Artemia salina