Producción CyT
Libro de abstract - TORQUE TENO VIRUS (TTV) VIRAL LOAD KINETICS AND THE ASSOCIATION WITH GRAFT REJECTION IN RENAL TRANSPLANT PATIENTS
Congreso
Autoría:
Reyes Noelia Soledad ; Jara Raquel ; Boccia Natalia ; GONZALO GARCIA ; Hermida, Eliana ; Diaz Carlos ; Soler Pujol Gervasio ; Poletta, Fernando Adrian ; Laham, Gustavo ; ECHAVARRIA, MARCELA SILVIAFecha:
2023Editorial y Lugar de Edición:
ASMResumen *
BackgroundRenal transplantation is the standard treatment for patients with end-stage renal disease. Graftsurvival is mainly determined by rejections and infectious complications in transplant recipients.Torque Teno Virus (TTV), a nonpathogenic and ubiquitous ssDNA virus, has been proposed as abiomarker of the immune status in transplant patients.Objectives: to determine the correlation between a TTV Home Brew PCR and a commercial PCR (R-GENE®PCR), to determine TTV viral load kinetics in renal transplant recipients and theassociation with graft rejection.MethodsA prospective cohort study on adult renal transplant recipients, transplanted between November2018 and April 2021, is being conducted at CEMIC University Hospital, Argentina.TTV viral load was determined by Home Brew PCR and R-GENE®PCR.For graft rejection association, TTV viral load was determined in the last sample obtained beforethe graft rejection event.ResultsTTV viral load was determined in 746 plasma samples from 107 renal transplant patients.Agreement of both PCR assays was 93.2% and Pearson correlation coefficient was r: 0.902 (95%CI:0.888-0.915, p<0.0001).TTV viral load kinetics showed an initial gradual increase, reaching a peak at 3 months. This peakwas followed by a slight decrease, reaching a plateau at 6 months, that was significantly higher thanpre-transplant values (p<0.0001).Graft rejection occurred in 28/92 (30.4%) patients. Most were T-cell mediated rejections (75%),followed by borderline changes (18%) and antibody mediated rejections (7%).Between 3-6 months post-transplantation, TTV median viral load in patients with graft rejectionwas significantly lower, 3.6 Log 10 copies/mL (by Home Brew PCR) and 3.1 Log 10 copies/mL (by R-GENE®PCR), compared to patients with no graft rejection (6.1 and 6.0 Log 10 copies/mL, respectively)(p=0.047 and p=0.012).ConclusionsA high TTV viral load correlation was observed between Home Brew PCR and a commercial PCR (R-GENE®PCR).TTV viral load showed dynamics kinetics post transplantation.Graft rejection was high in this population, being T-cell mediated rejection the most frequent type.At 3-6 month, TTV viral load was significantly lower in patients with renal rejection compare topatients with no graft rejection. Información suministrada por el agente en SIGEVAPalabras Clave
RENAL TRANSPLANTTTVBIOMARKERGRAFT REJECTION