Producción CyT
LI Reunión Anual de la Sociedad Argentina de Biofisica: libro de resúmenes - Antibacterial Action of T. Minuta Essential Oil: Insights into Membrane Effects
Congreso
Fecha:
2023Editorial y Lugar de Edición:
Sociedad Argentina de BiofísicaISSN:
978-987-48938-1-9Resumen *
Tagetes minuta L., popularly known as Chinchilla, is a native South American plant that has been used for different applications, including traditional medicine for antispasmodics, antiseptics, and stomach disorders, among others. It has been reported that the essential oil (EO) obtained from T. minuta shows interesting biological activities as an antifungal, antiviral, antibacterial, and insecticide. In this context, the objectives of this work were to obtain by hydrodistillation the EO from the leaves of this species located in Santiago del Estero, and characterize its chemical composition and antibacterial action. The chemical profile was determined by gas chromatography coupled with mass spectrometry, indicating that the major components of the EO were dihydrotagetone, ocimene, and tagetone. The minimal inhibitory concentrations (MIC) and minimalbactericidal concentrations (MBC) against gram-positive S. aureus ATCC 252923 and gram-negative E. coli ATCC 252922 were obtained. This EO has shown a MIC of 10 μg/mL and 20 μg/mL for S. aureus and E. coli, respectively. On the other hand, CBM has only been observed in E. coli at the maximal concentration tested, 20 μg/mL. By fluorescence microscopy studies, it has been shown that after 24 h of incubation with this EO, lead permeability changes in the membranes of both bacteria at the MIC concentration. This fact indicates that the bacterial membrane represents an important target for the antimicrobial action observed. In this sense, the change in melting transition temperature (Tm) by dynamic scattering light and laurdan fluorescence polarization (GP) by the action of EO in the biomimetic membrane system, liposomes of DMPC and DMPG (5:1) were determined. The EO-membrane interaction reduces the Tm and the cooperative interaction between phospholipid chains and decreases the laurdan GP. These results indicate that the EO is capable of affecting the membrane, increasing fluidity and the access of molecular water, confirming that the bacterial membrane is one of the targets for the antibacterial action. Información suministrada por el agente en SIGEVAPalabras Clave
AntimicrobialFluiditymembraneEssential oil