Producción CyT

Antimicrobial activity of the Lacticaseibacillus rhamnosus CRL 2244 and its impact on the phenotypic and transcriptional responses in carbapenem resistant Acinetobacter baumannii

Artículo

Autoría:

Cecilia Rodriguez ; Dema Ramlaoui ; Nardin Georgeos ; Briea Gasca ; Camila Leal ; Tomás Subils ; Marisel R. Tuttobene ; SIEIRA, RODRIGO ; Nicholas T. Salzameda ; Robert A. Bonomo ; Raúl Raya ; María Soledad Ramirez

Fecha:

2023

Editorial y Lugar de Edición:

Nature Research

Revista:

Scientific Reports, vol. 13 Nature Research

Resumen *

Carbapenem-resistant Acinetobacter baumannii (CRAB) is a recognized nosocomial pathogen with limited antibiotic treatment options. Lactic acid bacteria (LAB) constitute a promising therapeutic alternative. Here we studied the antibacterial properties of a collection of LAB strains using phenotypic and transcriptomic analysis against A. baumannii clinical strains. One strain, Lacticaseibacillus rhamnosus CRL 2244, demonstrated a potent inhibitory capacity on A. baumannii with a significant killing activity. Scanning electron microscopy images showed changes in the morphology of A. baumannii with an increased formation of outer membrane vesicles. Significant changes in the expression levels of a wide variety of genes were also observed. Interestingly, most of the modified genes were involved in a metabolic pathway known to be associated with the survival of A. baumannii. The paa operon, Hut system, and fatty acid degradation were some of the pathways that were induced. The analysis reveals the impact of Lcb. rhamnosus CRL 2244 on A. baumannii response, resulting in bacterial stress and subsequent cell death. These findings highlight the antibacterial properties of Lcb. rhamnosus CRL 2244 and its potential as an alternative or complementary strategy for treating infections. Further exploration and development of LAB as a treatment option could provide valuable alternatives for combating CRAB infections. Información suministrada por el agente en SIGEVA

Palabras Clave

ACINETOBACTERLACTICASEIBACILLUSANTIMICROBIAL ACTIVITYCARBAPENEM-RESISTANT