Producción CyT
Congreso
Autoría
Fecha
2017
Editorial y Lugar de Edición
Sociedad Argentina de Investigaciones Clínicas
Resumen
Información suministrada por el agente en
SIGEVA
Reports about antioxidant effect on obesity are controversial. We showed that N-acetylcysteine (NAC) inhibits cellular lipid accumulation during 3T3-L1 adipocyte differentiation, through the inhibition of adipogenic transcription factors expression, such as PPARγ and, MAPK phosphorylation (Soto el al, Redox Rep 2016). Objectives: Here, we evaluated NAC effect on fully differentiated 3T3-L1 adipocytes (AC) and developed nanoparticles with NAC. Methods: Assays were conducted using AC, diffe...
Reports about antioxidant effect on obesity are controversial. We showed that N-acetylcysteine (NAC) inhibits cellular lipid accumulation during 3T3-L1 adipocyte differentiation, through the inhibition of adipogenic transcription factors expression, such as PPARγ and, MAPK phosphorylation (Soto el al, Redox Rep 2016). Objectives: Here, we evaluated NAC effect on fully differentiated 3T3-L1 adipocytes (AC) and developed nanoparticles with NAC. Methods: Assays were conducted using AC, different doses of NAC (0.01 to 5 mM) were added to culture media for 5 days and, we determined: cellular content of triglycerides (Tg) and Oil-Red-O stained lipids (ORO), NAC toxicity by MTT assay, PPARγ protein expression by western blot assay, mRNA levels of PPARγ and lipid protein perilipin (Pl) by RT-qPCR analysis. NAC content in nanoparticles was determined by Raggi method. Results: Treatments with 0.01 to 5 mM NAC were not toxic on AC. 5mM NAC treatment on AC (ACN) elicited a 60% decrease in cellular Tg content (1.22+0.09 gTg/g protein [AC] vs 0.49+0.03 gTg/g protein [ACN], p<0.05). We evaluated ORO content in ACN compared to AC, which is set to 100 (100+4 [AC] vs 80+2 [ACN] arbitrary units (AU), p< 0.05), mRNA levels of Pl (Pl/ Rplp0:100+13 [AC] vs 72+9 [ACN] AU, p< 0.05) and, expression of PPARγ protein (PPARγ/GAPDH:100+6 [AC] vs 70+4 [ACN] AU; p< 0.05) and mRNA (PPARγ/Rplp0:100+7 [AC] vs 74+14 [ACN] AU, p<0.05). We developed silica hollow nanoparticles (100nm diameter, Nano) including 5mM NAC, as a better tool for NAC administration. We evaluated 5 different Nano preparations; our results showed a maximum of 13.2% incorporation of the total NAC. Conclusions: 5 mM NAC treatment on AC produced an effectively decrease in Tg content, and 20-30% decrease in ORO, Pl and PPARγ cellular contents. These results suggest that NAC could inhibit new lipid production in adipocytes. We also developed nanoparticles with NAC to use on AC.Key words: adipocytes, antioxidant, obesity
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Palabras Clave
CELL CULTUREADIPOCYTESANTIOXIDANTS