Producción CyT
European Society for Clinical Virology, Libro de resumenes - High TTV species diversity and variability in renal transplant recipients
Congreso
Autoría:
REYES, NOELIA SOLEDAD ; Spezia, Pietro G. ; Jara, Raquel ; Filippini Fabio ; Boccia, Natalia ; Garcia, Gonzalo ; Hermida Eliana ; Diaz, Carlos ; Soler Pujol, Gervacio ; Poletta Fernando ; Pistello Mauro ; Maggi Fabrizio ; Echavarria, MarcelaFecha:
2023Editorial y Lugar de Edición:
ESCV 2023Resumen *
IntroductionTorque Teno Virus (TTV) is a nonpathogenic and ubiquitous ssDNA virus, member of the Anelloviridae family, classified into 22 species (including more than 100 different strains). Objective: to determine TTV species diversity in renal transplant patients and to evaluate species variability within the same host.MethodsPlasma samples from recipients who underwent renal transplantation at CEMIC University Hospital were prospectively evaluated for TTV viral load before and during the first year after transplantation. TTV was detected using two assays (Home-Brew PCR and R-GENE®PCR). TTV typing was performed by whole genome sequencing. ORF-1 phylogenetic analysis was performed using the Maximum Likelihood method. Species variability was determined before and after transplantation.ResultsA total of 107 patients were enrolled from November 2018 to April 2021. All patients were TTV positive at some point after transplantation. At the pre-transplant period, TTV median viral loads were 3.0 and 2.8 Log10 copies/mL with Home-Brew PCR and R-GENE®PCR, respectively. At month 3, median viral loads were 6.8 Log10 copies/mL (Home-Brew PCR) and 6.1 Log10 copies/mL (R-GENE®PCR).A total of 30 plasma samples from 27 patients -randomly selected- were successfully typed. Overall, 16 different species were detected.Most prevalent species were TTV3 (63%) and TTV24 (50%), followed by TTV18 (43%) and TTV13 (43%).Median number of TTV species per patient was 4 (IQR: 2-6.3). Up to 10 TTV species were detected in one patient. In the pre-transplant period, 12/30 (40%) samples showed the highest median number of TTV species per sample, 5 (IQR: 4-7.5). At month 3, median TTV species per sample was 3.5 (IQR: 2-7).Variability: the post-transplant sample from three patients showed a decrease in the number of TTV species compared to the pre-transplant sample. Specifically, 2/3 had different TTV species. In addition, 1/3 patient’s post-transplant sample had Torque Teno Mini Virus (TTMV).ConclusionsHigh TTV species diversity was documented in renal transplant patients, especially in the pre-transplant period.Higher TTV species diversity was not associated with a higher TTV viral load.Total species variability within the same host was detected. Información suministrada por el agente en SIGEVAPalabras Clave
anellovirusesSpecies TTVAnelloviriridae TTV