Producción CyT

MEDICINA - Ischemic stroke induces mild acute left ventricular dysfunction and ECG abnormalities in a mousse model of brain ischemia/reperfusion.

Congreso

Autoría:

Barbieri I ; FRANCO RIVEROS, VERENA BEATRIZ ; Casanova V ; Buchholz B

Fecha:

2023

Editorial y Lugar de Edición:

Fundación Revista Medicina

Resumen *

Objective: The aim was to determine if a transient ischemic stroke induces cardiac dysfunction through autonomic imbalance, due to sympathetic overactivity. Materials and Methods: Mice underwent transient middle cerebral artery occlusion (tMCAo) for 60 min and 24h reperfusion. Gsalpha transgenic mice (TG) were used as a model of basal sympathetic hyperactivity and were also exposed To tMCAo (I/R-TG). Left ventricular function (LVF) was assessed by echocardiography: ejection fraction (EF), shortening fraction (SF) and isovolumetric relaxation time (IVRT). Electrocardiography (ECG) was performed throughout the ischemia and after 24h of reperfusion, to assess electric abnormalities. Infarct size (IS) was measured by TTC stain after 24h reperfusion. Neurological deficit was assessed 4h and 24h after tMCAo using Longa's scale (LS). Mortality was also assessed and compared between groups. Results: tMCAo produced similar IS in both experimental groups (I/R- NTG: 30.0 ± 4.3%, I/R-TG: 26.2 ± 5.0%; p=NS). We observed LV dysfunction, characterized by a mild decrease in EF in NTG mice (I/R-NTG: 72.5±1.8% vs SHAM NTG: 87.4±1.0% p<0.05) and TG mice (I/R-TG: 81.4±1.5% vs SHAM TG: 87.3±1.3% p<0.05) and SF in NTG mice (I/R-NTG: 42.6±1.9% vs SHAM NTG: 51.3±1.8% p<0.05) and TG mice (I/R-TG: 44.8±1.4% vs SHAM TG: 49.8±1.5% p=NS). IVRT significantly decreased in the TG group (I/R-TG: 18.1±0.7ms vs I/R-NTG: 20.8±0.7ms; p<0.05). Prolonged QTc interval was observed after 60 min of ischemia (157.4±5.8ms) and persisted after 24h reperfusion (158.9±2.9ms) with respect to basal (129.2±5.2ms; p<0.05) in the I/R-TG group. After 24h of reperfusion, 42.9% of the NTG mice survived, compared to 26.7% of TG mice (p<0.05). Conclusion: tMCAo induces an acute mild LV dysfunction and ECG abnormalities after 24h reperfusion. A significant increase in the mortality rate was observed in TG mice. Información suministrada por el agente en SIGEVA

Palabras Clave

ECG ABNORMALITIESISCHEMIC STROKEMICEBRAIN ISCHEMIA/REPERFUSION