Producción CyT

Medicina - HEMEOXYGENASE-1 PLAYS A PROTUMORAL ROLE IN THYROID CANCER

Congreso

Autoría:

ALONSO, EXEQUIEL GONZALO ; Pichel, P. ; Mascaró, M. ; Schweitzer, K. ; Fernandez Chavez, L. ; Coló, G.P. ; Recio, S. ; Rosemblit, C. ; Facchinetti, M.M. ; Curino, A.C.

Fecha:

2021

Editorial y Lugar de Edición:

Medicina

ISSN:

1669-9106

Resumen *

We have previously demonstrated that hemeoxygenase-1 (HO-1) mRNA is overexpressed in papillary (PTC) and anaplastic (ATC) thyroid tumor compared to non-malignant areas to the tumor (NMT). We also demonstrated that HO-1 protein levels are up-regulated in human PTC samples, showing cytoplasmic localization, and such HO-1 tumor expression correlates with histological subtype. Now, we aim to study the role of HO-1 in thyroid cancer (TC) biology. To that end, we evaluated HO-1 expression by indirect immunofluorescence, cell viability by crystal violet method, cell cycle progression by propidium iodide staining and flow cytometry, and cell migration by wound healing assay. We found that pharmacological activation of HO-1 using 80μM hemin increased cell viability in TPC-1 (p<0.001) and 8505c (p<0.001) cell lines at 72h. In TPC-1, we found that hemin increased cell number in S- (p<0,05) and G2/M (p<0,001) phases and diminished cell number in Go/G1 phase (p<0,001) at 48h. In 8505c, hemin increased cell number in S- (p<0,001) phase and diminished cell number in Go/G1 (p<0,01) and G2/M (p<0,001) phases at 48h. In Nthy-Ori-3-1, a normal thyroid cell line, we found that 80μM hemin decreased (p<0.001) cell viability at 72h. Also, we found that 80μM hemin increased cell migration (p<0.001) in TPC-1 and 8505c cell lines. On the contrary, inhibition of HO-1 activity using 16μM ZnPP decreased cell viability in TPC-1 (p<0.001) and 8505c (p<0.001) cell lines at 72h while no differences were observed in Nthy-Ori-3-1 cells. In TPC-1, ZnPP diminished cell number in Go/G1 phase (p<0,01) and increased cell number in G2/M (p<0,05) at 48h. However, in 8505c, cell cycle progression remained unaltered after ZnPP treatment. Also, 16μM ZnPP reduced TPC-1 cell migration (p<0.001), but in 8505c ZnPP failed to alter migratory capacity. In conclusion, our results demonstrate that HO-1 plays a protumoral role in TC cells by altering cell survival, cell cycle progression and cell migration. Información suministrada por el agente en SIGEVA

Palabras Clave

HEMEOXYGENASE-1CANCERTHYROID