Producción CyT

Medicina - Antioxidant activity of atrial natriuretic peptide (ANP) in the exocrine pancreas

Congreso

Autoría:

Courreges, Ana Paula ; ALVAREZ, GUADALUPE INÉS ; Ochoa, Federico ; Zotta, Elsa ; Repetto, Marisa ; Vatta, Marcelo ; Bianciotti, Liliana

Fecha:

2022

Editorial y Lugar de Edición:

Medicina

ISSN:

1669-9106

Resumen *

ANTIOXIDANT ACTIVITY OF ATRIAL NATRIURETI PEPTIDE (ANP) IN THE EXOCRINE PANCREASCourreges, Ana P, Alvarez Guadalupe I, Ochoa Federico, Lairion Fabiana, Zotta Elsa, Repetto Marisa, Vatta Marcelo S , Bianciotti Liliana G. We previously reported that ANP is produced by the exocrine pancreas and that it plays a beneficial role in the outcome of acute pancreatitis (AP). It reduces trypsinogen activation and the inflammatory response and restores glutathione depletion. In the present study we assessed different parameters of oxidative stress and antioxidant defenses in Sprague Dawley rats with AP induced by four repetitive cerulein injections (40μg/Kg). Thirty minutes before the first cerulein injection animals were infused with either saline (control) or ANP (1μg/Kg/h) for 60 min. Following euthanasia (60 min after the last cerulein injection) pancreatic samples were harvested. ANOVA followed by a Student Newman Keuls was used for statistical analysis. Results are expressed as the mean±SD and p<0.05 or less were considered statistically significant (*). ANP reduced NADPH oxidase activity (U/ug protein) (2180±256 vs. 1410±216**). As ANP increased Nrf2 nuclear translocation (0.158±0.2 vs. 0.398±0.02*) superoxide dismutase (SOD) and catalase expression were assessed by western blot and qRT-PCR. ANP increased SOD protein and mRNA expression (0.9±0.08 vs; 1.20±0.11*; 0.63±0.12 vs. 2.29±0.19*). Catalase showed no protein or mRNA changes as expected. Carbonyl content was decreased by ANP (5.60±0.6 vs. 3.60±0.9*) and TBARS showed no changes in any experimental group. The oxidized form of coenzyme Q9 (isoform in rodents) was also reduced by ANP (477.9±56.9vs. 254.7±86.4*). These results further support previous findings showing that ANP enhances the antioxidant capacity of the pancreas in AP. Current evidence suggests that targeting only oxidative stress would not be sufficient to stop the progression of AP, a pathology characterized by a sudden onset and an unpredictably clinical course. In this sense ANP, which it is produced in the exocrine pancreas, would be beneficial since it not only improves the redox status of the pancreas but also significantly reduces premature trypsinogen activation and the local inflammatory response Información suministrada por el agente en SIGEVA

Palabras Clave

ANPPáncreas exógenoAntioxidante