Producción CyT
IMBS Abstract Book - The absence of CB1R induces alterations in neuronal cytoarchitecture.
Congreso
Fecha:
2016Editorial y Lugar de Edición:
FFYBResumen *
The cannabinoid receptor type 1 (CB1R) is widely distributed in the central nervous system and is involved in physiological processes such as memory, learning, motor coordination, anxiety and mood. Cannabinoid receptors are expressed in hippocampus. The CB1R is preferentially expressed in pyramidal neurons. CB1R is mainly located on presynaptic neurons and modulates synaptic activity. Genetic deletion of CB1R causes behavioral alterations similar to those described in animal models of depression but the morphological substrate of this behavior is not fully known. The study was performed by immunofluorescence, Golgi, Western Blot and electron microscopy techniques.Undifferentiated neuronal cells precursors (Nestine + cells) decreases in number in CB1R-/- mice, and also the number of migrating neuroblasts (doublecortin +). This data suggests that adult neurogenesis, in dentate gyrus, are altered in this type of mice.CB1R-/- mice showed a decrease in the expression of the neuronal cytoskeleton components (Nf-160kDa, Nf-200kDa and MAP-2). We also observed a reduction in the number of primary projections. In addition, results showed a decrease in Synaptophysin expression, and a tendency to increase the number of vesicles in the synaptic terminal in CB1R-/- mice, probable evidencing changes in neurotransmitter release. The number of synapses was not affected but the postsynaptic density thickness was reduced.The absence of CB1R alters neuronal architecture in the adult brain, affecting synaptic plasticity and modeling. Such changes might correlate with reported changes in the behavior in CB1R-/- mice. Información suministrada por el agente en SIGEVAPalabras Clave
neuronal plasticitycb1cannabinoids