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CASTELLO, PABLO RAUL

INVESTIGADOR INDEPENDIENTE

SPECIALITY:

Nutraceutico. Hipoxia. Proteinas de membrana. Especies Reactivas del oxigen y Nitrogeno

Scientific discipline:

Bioquímica y Biología Molecular - Biología

Topic:

Fideos nutracéuticos a base de complejos tipo V de almidones modificados/polifenoles obtenidos in situ por extrusión . Nutraceutical noodles based on type V complexes of modified starches/polyphenols obtained in situ by extrusion

Workplace

DEPARTAMENTO DE QUIMICA Depends on
  • UNIVERSIDAD DE BELGRANO (UB)
    • FACULTAD DE CS.EXACTAS Y NATURALES
Address:
VILLANUEVA 1324, 1426 - Capital Federal - Argentina

Contact:

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S&T Expertise*

Cell Culture: Cell culture experience with yeast and bacteria. Expert fermentor operator.Cell culture experience with mammalian cell lines and primary cell cultures, transient and stable transfections, adenoviral and retroviral infections. DNA, RNA: RNA extraction, RNA quantification (Q-PCR), Northern blots. RNA and DNA manipulation. Molecular cloning, minipreps, maxipreps, gel shiftsMitochondria: More than 8 years of working with mitochondria isolation from several sources includind tissues and cells., Mitochondria bioenergetics, respiration and oxidative phosphorylation analysis, production of free radicals, measurement of membrane potential, mitochondria engineering, protein analysis, by several methods, enzyme analysis, measurement of cytochromes interaction with xenobiotics, isolation of mitochondria and subfractionation of mitochondriaProteins: Strong background in cloning, expression and protein purification from natural and recombinant sources using several techniques: differential centrifugation, chromatography (FPLC, ion-exchange, affinity, size exclusion, HPLC). *Information provided by the agent in SIGEVA

Lines of Investigation

Modulacion de radicales libres por factores ambientales CIENCIAS NATURALES Y EXACTAS - Ciencias Biológicas - Biofísica

Technological Capacities

  • 5. Ciencias físicas y exactas
    • 1. Química
      • 1.1. Química analítica
      • 1.3. Química inorgánica
      • 1.4. Química orgánica
    • 4. Tecnologías de separación
      • 4.1. Procesos de filtración y con membranas
      • 4.2. Extracción
      • 4.3. Absorción
      • 4.4. Destilación
  • 6. Ciencias biológicas
    • 2. Biología / Biotecnología
      • 2.1. Bioquímica / biofísica
      • 2.2. Biología celular y molecular
      • 2.3. Ingeniería genética
      • 2.4. Ensayos in vitro, experimentos

Key Words

MitocondriaHipoxiaRadicales libresFree ReadicalsCampos MagneticosMitochondriaHypoxiaMagnetic fields

Education

1992 - 1998

Doctor de la Universidad de Buenos Aires

UNIVERSIDAD DE BUENOS AIRES

1984 - 1994

Bioquimico

UNIVERSIDAD DE BUENOS AIRES

1984 - 1989

Farmaceutico

UNIVERSIDAD DE BUENOS AIRES


S&T HR Training

Co-director of

Science and Technology Production

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