Science and Technology Production
Women in Autophagy (WIA) Beth Levine?s Legacy Network 1st Annual Symposium - Roles of autophagy in growth and development in the moss P. patens.
Congreso
Authorship:
PETTINARI G ; FINELLO J ; ROBERT G ; LASCANO R ; SAAVEDRA LDate:
2020Publishing House and Editing Place:
Women in Autophagy (WIA)Summary *
Autophagy is an evolutionary conserved cellular process that involves sequestration,degradation and recycling of cytoplasmic components, thus constituting a fundamentalmechanism for the maintenance of cellular homeostasis in eukaryotic organisms. In the last decade autophagy research has flourished, pointing out that it is virtually involved in all aspects of plant physiology. Most knowledge and major contributions in the field of plant autophagy arise from studies in seed plants. However, in the last years availability of sequenced genomes from several lineages of streptophyte algae and bryophytes, allowed us to dive into evolutionary aspects of plant autophagy as well as specie‐specific roles of this mechanism. In particular, our research is focused on bryophytes the earliest diverging group of terrestrial flora, and use the moss Physcomitrium patens as model system.In this study, we show that the core Autophagy (ATG) machinery is present in P. patens. We generated atg5 and atg7 knock out lines and found that the protective role of autophagy under nutrient deficiency (darkness and nitrogen starvation) is conserved in P. patens. The presence of different cell types with apical growth in P. patens, offers the possibility to stud ythe interaction between this process and autophagy. Differences observed in protonemata cells of atg mutants and expression analysis of PpATG8 genes, together with quantification analysis of autophagic vesicles using an autophagy reporter moss line (PpATG8b::GFPPpATGb),provide evidences for the role of autophagy to sustain the apical growth of protonemata during the darkness under optimal growth conditions. Information provided by the agent in SIGEVAKey Words
autophagynutritional deficiencyatg5 and atg7Physcomitrium patens