Science and Technology Production
Article
Authorship
Franco M. Cabrerizo
;
Andrés H. Thomas
;
LORENTE, CAROLINA
;
M. Laura Dántola
;
Gabriela Petroselli
;
Rosa Erra-Balsells
;
Alberto L. Capparelli
Date
2004
Publishing House and Editing Place
WILEY-V C H VERLAG GMBH
Magazine
HELVETICA CHIMICA ACTA,
vol. 87
(pp. 349-365)
WILEY-V C H VERLAG GMBH
Summary
Information provided by the agent in
SIGEVA
Photochemical studies of the reactivity of 6-(hydroxymethyl)pterin (2-amino-6-(hydroxymethyl)pteridin- 4(1H)-one; HPT) in alkaline aqueous solutions (pH 10.2 ± 10.8) at 350 nm and room temperature were performed. The photochemical reactions were followed by UV/VIS spectrophotometry, thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), and an enzymatic method for H2O2 2-amino-6-(hydroxymethyl)pteridin- 4(1H)-one; HPT) in alkaline aqueous solutions (pH 10.2 &plus...
Photochemical studies of the reactivity of 6-(hydroxymethyl)pterin (2-amino-6-(hydroxymethyl)pteridin- 4(1H)-one; HPT) in alkaline aqueous solutions (pH 10.2 ± 10.8) at 350 nm and room temperature were performed. The photochemical reactions were followed by UV/VIS spectrophotometry, thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), and an enzymatic method for H2O2 2-amino-6-(hydroxymethyl)pteridin- 4(1H)-one; HPT) in alkaline aqueous solutions (pH 10.2 ± 10.8) at 350 nm and room temperature were performed. The photochemical reactions were followed by UV/VIS spectrophotometry, thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), and an enzymatic method for H2O2 H)-one; HPT) in alkaline aqueous solutions (pH 10.2 ± 10.8) at 350 nm and room temperature were performed. The photochemical reactions were followed by UV/VIS spectrophotometry, thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), and an enzymatic method for H2O22O2 determination. In the presence of O2 , 6-formylpterin (2-amino-3,4-dihydro-4-oxopteridine-6-carboxaldehyde; FPT) was the only photoproduct detected. In the absence of O2 , we observed a compound with an absorbance maximum at 480 nm, which was oxidized very rapidly by O2 in a dark reaction to yield FPT. The quantum yields of substrates disappearance and of photoproducts formation were determined. The formation of H2O2 during photooxidation was monitored, and the number of mol of H2O2 released per mol of HPT consumed corresponded to a 1 :1 stoichiometry. HPTwas also investigated for efficiency of singlet-oxygen (1O2) production and quenching in aqueous solution. The quantum yield of 1O2 production ( 2 , 6-formylpterin (2-amino-3,4-dihydro-4-oxopteridine-6-carboxaldehyde; FPT) was the only photoproduct detected. In the absence of O2 , we observed a compound with an absorbance maximum at 480 nm, which was oxidized very rapidly by O2 in a dark reaction to yield FPT. The quantum yields of substrates disappearance and of photoproducts formation were determined. The formation of H2O2 during photooxidation was monitored, and the number of mol of H2O2 released per mol of HPT consumed corresponded to a 1 :1 stoichiometry. HPTwas also investigated for efficiency of singlet-oxygen (1O2) production and quenching in aqueous solution. The quantum yield of 1O2 production ( 2 , we observed a compound with an absorbance maximum at 480 nm, which was oxidized very rapidly by O2 in a dark reaction to yield FPT. The quantum yields of substrates disappearance and of photoproducts formation were determined. The formation of H2O2 during photooxidation was monitored, and the number of mol of H2O2 released per mol of HPT consumed corresponded to a 1 :1 stoichiometry. HPTwas also investigated for efficiency of singlet-oxygen (1O2) production and quenching in aqueous solution. The quantum yield of 1O2 production ( 2 in a dark reaction to yield FPT. The quantum yields of substrates disappearance and of photoproducts formation were determined. The formation of H2O2 during photooxidation was monitored, and the number of mol of H2O2 released per mol of HPT consumed corresponded to a 1 :1 stoichiometry. HPTwas also investigated for efficiency of singlet-oxygen (1O2) production and quenching in aqueous solution. The quantum yield of 1O2 production ( 2O2 during photooxidation was monitored, and the number of mol of H2O2 released per mol of HPT consumed corresponded to a 1 :1 stoichiometry. HPTwas also investigated for efficiency of singlet-oxygen (1O2) production and quenching in aqueous solution. The quantum yield of 1O2 production ( 2O2 released per mol of HPT consumed corresponded to a 1 :1 stoichiometry. HPTwas also investigated for efficiency of singlet-oxygen (1O2) production and quenching in aqueous solution. The quantum yield of 1O2 production ( 1O2) production and quenching in aqueous solution. The quantum yield of 1O2 production (1O2 production ( 0.210.01) was determined by measurements of the 1O2 luminescence in the near-IR (1270 nm) upon continuous excitation of the sensitizer. The rate constant of 1O2 total quenching by HPT was determined (kt3.1 ¥ 106 1 s1), indicating that this compound was able to quench 1O2 . However, 1O2 did not participate in the photooxidation of HPT to FPT. 0.210.01) was determined by measurements of the 1O2 luminescence in the near-IR (1270 nm) upon continuous excitation of the sensitizer. The rate constant of 1O2 total quenching by HPT was determined (kt3.1 ¥ 106 1 s1), indicating that this compound was able to quench 1O2 . However, 1O2 did not participate in the photooxidation of HPT to FPT. 1O2 luminescence in the near-IR (1270 nm) upon continuous excitation of the sensitizer. The rate constant of 1O2 total quenching by HPT was determined (kt3.1 ¥ 106 1 s1), indicating that this compound was able to quench 1O2 . However, 1O2 did not participate in the photooxidation of HPT to FPT. 1O2 total quenching by HPT was determined (kt3.1 ¥ 106 1 s1), indicating that this compound was able to quench 1O2 . However, 1O2 did not participate in the photooxidation of HPT to FPT.1O2 . However, 1O2 did not participate in the photooxidation of HPT to FPT.
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Key Words
PTERINSPHOTOCHEMISTRY