Science and Technology Production
Article
Authorship
Date
2010
Publishing House and Editing Place
Inderscience Enterprises
Magazine
Int J. Low Radiation,
vol. 7
(pp. 366-379)
- ISSN 1477-6545
Inderscience Enterprises
Inderscience Enterprises
ISSN
1477-6545
Summary
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SIGEVA
Objective: To study the application of PTU as a radioprotector in the thyroid gland. Methods and Materials: Rat thyroid epithelial cells (FRTL-5) and human colon cancer cells (ARO81-1) were exposed to ã-irradiation with or without 1 mM PTU. Radiation response was analyzed by clonogenic survival assay. Cyclic AMP levels were measured by radioimmunoassay (RIA). Results: PTU increased surviving cell fraction (SF) at 2 Gy significantly (p<0.05) in both cell lines. PTU increased extra cell...
Objective: To study the application of PTU as a radioprotector in the thyroid gland. Methods and Materials: Rat thyroid epithelial cells (FRTL-5) and human colon cancer cells (ARO81-1) were exposed to ã-irradiation with or without 1 mM PTU. Radiation response was analyzed by clonogenic survival assay. Cyclic AMP levels were measured by radioimmunoassay (RIA). Results: PTU increased surviving cell fraction (SF) at 2 Gy significantly (p<0.05) in both cell lines. PTU increased extra cellular levels of cAMP in all the treatments in a dose and time dependent manner for FRTL-5 cells. In ARO81-1 cells a peak was observed at 24 hs in extracellular levels incubated with 1 mM PTU (36.97 ± 6.74 fmol/µg prot vs control: 17.53 ± 3.9 fmol/µg prot, p<0.001). Forskolin and dibutyril cAMP mimicked the effect of PTU on SF. Discussion: PTU is a radioprotector for thyroid cells and could exert its effect through cAMP.
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Key Words
THYROIDRADIOPROTECTIONPTURADIATION