Science and Technology Production
Congress
Authorship
BLANCO, NICOLAS ERNESTO
;
Poli, H.O.
;
Valle,E.M.
;
Carrillo, N.
Date
2005
Summary
Information provided by the agent in
SIGEVA
Ferredoxins (Fd) are a group of low molecular-weight proteins which have Fe-S centres. Plant-type Fds, that have one 2Fe-2S cluster per molecule, acts as a soluble electronic transporters between Photosystem I and ferredoxin-NADPH reductase (FNR) during the light phase of photosynthesis, to generate NADPH needed for CO2 fixation. Moreover, there are other Fd-depedent enzymes, in which the reducing equivalents are utilized for nitrogen and sulphur assimilation and for nitrogen fixation. We have ...
Ferredoxins (Fd) are a group of low molecular-weight proteins which have Fe-S centres. Plant-type Fds, that have one 2Fe-2S cluster per molecule, acts as a soluble electronic transporters between Photosystem I and ferredoxin-NADPH reductase (FNR) during the light phase of photosynthesis, to generate NADPH needed for CO2 fixation. Moreover, there are other Fd-depedent enzymes, in which the reducing equivalents are utilized for nitrogen and sulphur assimilation and for nitrogen fixation. We have developed transgenic plants with different levels of ferredoxin. Plants with reduced levels of this protein were obtained using antisense technology and silencing using RNAsi. These plants showed variegated distribution of chlorophyll in the leaf, reduced CO2 fixation rates and lower tolerance to moderate oxidative conditions. Arrested growth, pale green leaves and incapability to generate offspring were observed in seedlings with extreme phenotypes generated by RNAsi silencing. On the other side, plants with higher than physiological levels of ferredoxin did not show striking differences when were compared with wild-type ones. All these results allow us to suggest that a small reduction in the levels of Fd would lead to drastic alterations in the growth and development of plants.
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