Evaluation of the different procedures in the detection of anti RO/SS-A 52 kDa and anti-RO/SS-A 60 kDa antibodies in primary Sjögren’s syndrome

Science and Technology Production

Article

Authorship

Daniel H. González Maglio ; PAZ, MARIELA LAURA ; N. Saccodossi ; C. Sgarlata ; C. Abatangelo ; L. Squiquera ; M. Fonseca ; Juliana Leoni

Date

2003

Publishing House and Editing Place

Sociedad Española de Inmunología

Magazine

Inmunología, vol. 22 (pp. 10-16) - ISSN 0213-9626
Sociedad Española de Inmunología

ISSN

0213-9626

Summary Information provided by the agent in SIGEVA

A group of patients with Sjögren’s Syndrome has been analysed in the present study in order to evaluate the different techniques used for the detection of anti Ro/SS-A of 52 and 60 kDa autoantibodies. The evaluated techniques were: double immunodiffusion performed in agarose gels using as antigen human spleen extract; Western Blot (WB) and enzyme-linked immune assays (ELISA) performed with recombinant protein antigens (Ro/SS-A 52 y 60 kDa in individual assays). Even though the ELISA ... A group of patients with Sjögren’s Syndrome has been analysed in the present study in order to evaluate the different techniques used for the detection of anti Ro/SS-A of 52 and 60 kDa autoantibodies. The evaluated techniques were: double immunodiffusion performed in agarose gels using as antigen human spleen extract; Western Blot (WB) and enzyme-linked immune assays (ELISA) performed with recombinant protein antigens (Ro/SS-A 52 y 60 kDa in individual assays). Even though the ELISA and WB techniques are more sensitive than the gel precipitation method, the latter cannot be completely replaced by the former. It is necessary to take into consideration the nature of the antigen used in order to draw conclusions. The recombinant antigens maintain the linear and conformational epitopes of the native molecules, but they are not able to bind to the other molecules of the RoRNP complex.On the other hand, in the antigenic extract used in the immunodiffusion technique, such interactions occur, and thus, the epitopes formed by the interaction of the different molecules might be present. Our results indicate that, in spite of their higher sensitivity, the ELISA and WB techniques are not capable of entirely replacing the immunodiffusion method.

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Key Words

AUTOANTIBODIESRO/SS-A 52 KDASJOGREN'S SYNDROMERO/SS-A 60 KDARIBONUCLEOPROTEINSLA/SS-B