Science and Technology Production
Biocell - Light, chromatin and alternative splicing
Congreso
Date:
2014Publishing House and Editing Place:
BiocellSummary *
Light is not only a source of energy but also a key regulator of plant physiological adaptations. We have previously shown that light/dark conditions affect alternative splicing of the pre-mRNA for At-RS31, a Ser-Arg-rich splicing factor. This led us to investigate whether chromatin modifications play a role in this light regulation of alternative splicing. Increasing concentrations of trichostatin A (TSA), a drug that suppresses histone deacetylase activity and therefore increases histone acetylation, mimic the effect of light on At-RS31 alternative splicing in a dose-dependent manner. The light/dark effect and the TSA effect on alternative splicing are not observed in the pre-mRNA for At-RS2Z33, another SR-splicing factor used as a control. Global histone acetylation does not seem to change between light and dark treated plants. Using Arabidopsis mutants defective in different histone deacetylases we found that the light/dark effect is strongly reduced in hd1 mutants. In contrast, the light/dark effect is still observed in hda15 mutants, suggesting a mechanism that involves specific histone modification enzymes. Further experiments such as a chromatin inmunoprecipitation assay of chromatin marks will be carried out in the future to determine the link between chromatin modifications and alternative splicing regulation by light. Information provided by the agent in SIGEVAKey Words
ALTERNATIVE SPLICINGLIGHTCHROMATIN