Science and Technology Production

Abstract Book . 10th Biometal . Symposium of Biodegradable Metals for Biomedical Aplications - Algorithms for the multidimensional microscopy images analysis. Application to study of macrophages-Fe based degradable biomaterials interactions

Congreso

Authorship:

Fagali, N ; Puntarulo, S ; Lozano Puerto, R ; Perez Maceda, B ; Fernández Lorenzo de Mele, M

Date:

2018

Publishing House and Editing Place:

Laval University

Summary *

INTRODUCTION: Advantages anddisadvantages of the use of pure iron (Fe) and someof its alloys for the manufacture of biodegradabletemporary stents have been discussed in the lastdecades. The evaluation of biocompatibility ?invivo? (in the area of the implant), is complex andthe use of cell cultures is a valuable model forpreliminary studies [1]. The analysis of theinteractions between cells and materials at real timeis helpful to provide information related to possibletoxic effects of Fe and its degradation productsformed after exposure to the biological media.RESULTS: Multidimensional microscopy (MDM)at real time was employed in this work to obtainimages and videos of live non-stained macrophagesin contact with bulk Fe. The acquired images werelater analyzed using different algorithms. TheMDM allows the study of the cell behavior inconditions where non-staining is required, avoidinga possible effect on cell metabolism.In order to study these interactions, murinemacrophages (J774A.1) were exposed to rings ofpure Feo(99.9% SpecPure) and their response wasanalyzed by MDM at real time. Images were takenevery 15 minutes for 24 and 48 hours, and then theframes were mounted as a video film.Several algorithms were designed for the analysis ofthe sequences of images using free software FIJIand different parameters were obtained such asnumber of live/dead cells, displacement, averagespeed, number of divisions (splitting) and othersmorphological parameters. It could be noticed thatthe speed, displacement and cell division werealtered with respect to control cells (in absence ofFe). As metal degrades, the macrophages captureand accumulate the degradation products as a darkdeposit of insoluble material and cells die. Information provided by the agent in SIGEVA

Key Words

MACROPHAGESIRON