Science and Technology Production
Article
Authorship
Volz, Pierre
;
SCHILRREFF, PRISCILA
;
Brodwolf, Robert
;
Wolff, Christopher
;
Stellmacher, Johannes
;
Balke, Jens
;
MORILLA, MARIA JOSE
;
Zoschke, Christian
;
Schäfer-Korting, Monika
;
Alexiev, Ulrike
Date
2017
Publishing House and Editing Place
Blackwell Publishing
Magazine
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES.,
vol. 1405
(pp. 202-214)
Blackwell Publishing
Summary
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Targeted topical application promises high drug concentrations in the skin and low systemic adverse effects. To locate drugs and drug?delivery systems like nanocarriers, fluorescent dyes are commonly used as drug surrogates or nanocarrier labels in micrographs of tissue sections. Here, we investigate how labeling degree, concentration of fluorophore, and nanocarrier may affect the interpretation of these micrographs. False?negative penetration results due to inter? and intramolecular quenching ...
Targeted topical application promises high drug concentrations in the skin and low systemic adverse effects. To locate drugs and drug?delivery systems like nanocarriers, fluorescent dyes are commonly used as drug surrogates or nanocarrier labels in micrographs of tissue sections. Here, we investigate how labeling degree, concentration of fluorophore, and nanocarrier may affect the interpretation of these micrographs. False?negative penetration results due to inter? and intramolecular quenching effects are likely. Using tecto?dendrimers as an example, we present a detailed analysis of pitfalls in the (semi?)quantitative evaluation of skin nanocarrier penetration. Fluorescence lifetime imaging microscopy (FLIM) allows distinguishing the target fluorescence of dye?tagged nanocarriers from skin autofluorescence, providing a highly sensitive tool for clear?cut localization of the nanocarriers. Cluster?FLIM images reveal that FITC?labeled tecto?dendrimers penetrate the stratum corneum of human skin ex vivo and reconstructed human skin but do not cross the tight junction barrier.
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Key Words
FLIMDRUG DELIVERY SYSTEMTIME-RESOLVED FLUORESCENCE SPECTROSCOPYBIOMACROMOLECULESTIGHT JUNCTIONSRECONSTRUCTED HUMAN SKIN
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