Science and Technology Production
Biophysical J - Effects of erythrocytes treated with alpha hemolysin of E.coli on endothelial cells?.
Congreso
Authorship:
Herlax Vanesa ; Leal Denis, María Florencia, ; Marginedas I ; Enrique, Nicolás; ; Sabina M. Maté. ; Veronica Milesi ; Ostuni, Mariano Anibal; ; Pablo J. SchwarzbaumDate:
2017Publishing House and Editing Place:
ELSEVIER SCIENCE BVSummary *
?Effects of erythrocytes treated with alpha hemolysin of E.coli on endothelial cells?Herlax, Vanesa1*; Leal Denis, María Florencia2,3, Marginedas Freixa, Irene 4,5 Enrique, Nicolás6,7; Mate, Sabina María1; Milesi, Verónica6,7, Ostuni, Mariano Anibal 4,5; and Schwarzbaum, Pablo Julio2,81- INIBIOLP-CONICET/UNLP, Facultad de Ciencias Médicas, Calle 60 y 120. La Plata, Argentina.2- IQUIFIB-CONICET/UBA. Junín 956. Buenos Aires, Argentina. 3- UBA. FFyB. Departamento de Química Biológica. Cátedra de Química Biológica Superior. Buenos Aires, Argentina. 4- INSERM. UMR_S1134. 6, rue Alexandre Cabanel. Paris, Francia. 5- Université Paris Diderot, Paris 7. Paris, Francia.6- IIFP-CONICET/UNLP. Calle 115 y 47. La Plata, Argentina. 7- UNLP. Exactas. Cátedra de Fisiología. La Plata, Argentina. 8- UBA. FFyB. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica. Buenos Aires, Argentina.* presenting authorUropathogenic strains of E. coli deliver the toxin alpha-hemolysin (HlyA) to optimize the host environment for the spread of infection. It was reported that at high concentrations, the toxin forms pores in eukaryotic membranes, leading to cell lysis, while lower concentrations might interfere with host-cell?signaling pathways, causing cell death by apoptosis. In the present investigation we demonstrate that a relatively low concentration of HlyA induces morphological changes and phosphatidylserine externalization of human erythrocytes. On the other hand, the unacylated nonhemolytic form of HlyA, ProHlyA induces similar morphological changes but no PS externalization. Since PS exposure of erythrocytes is known to induce cell adhesion, we used a dynamic cell adhesion platform to study the consequences of HlyA vs ProHlya exposure of erythrocytes on their adhesion to human endothelial cells (HMEC). Results indicate that HlyA-treated erythrocytes adhere more to endothelial cells than Pro-treated erythrocytes at low flux (0.5 din). At higher fluxes (1 and 2 din), however, HlyA-treated erythrocytes detached easily, indicating that the adherence is weak We also study the efflux of ATP from erythrocytes treated with both toxins by luciferin-luciferase luminescence. Results demonstrate that HlyA induces the efflux of ATP while ProHlyA does not. Since PS exposure was suggested to simultaneously increase extracellular ATP and adhesion to the vascular endothelium, and erythrocyte derived ATPe can alter the caliber of the vascular lumen, future experiments will be designed to relate HlyA induced efflux of ATP of erythrocytes with their adhesion and interaction with endothelial cells.Grants: ANPCyT (PICT 2013/2657), MINCYT- ECOS-Sud de Francia (A15S01) Information provided by the agent in SIGEVAKey Words
ATPHlyA