Science and Technology Production
Congress
Authorship
Date
2015
Publishing House and Editing Place
American Society for Microbiology
Summary
Information provided by the agent in
SIGEVA
Background: The globally distributed multidrug-resistant- K.pneumoniae ST258 have spread in our country since 2010, until they became endemic. However, in the last two years the emergences of sporadic KPC-producing- K. pneumoniae (KPCKp) retaining susceptibility to some antibiotics have been observed. The aim of this study was to perform a microbiological and epidemiological characterization of these sporadic KPCKp isolates recovered on a teaching Hospital. Methods:All KPCKp isolates recovered ...
Background: The globally distributed multidrug-resistant- K.pneumoniae ST258 have spread in our country since 2010, until they became endemic. However, in the last two years the emergences of sporadic KPC-producing- K. pneumoniae (KPCKp) retaining susceptibility to some antibiotics have been observed. The aim of this study was to perform a microbiological and epidemiological characterization of these sporadic KPCKp isolates recovered on a teaching Hospital. Methods:All KPCKp isolates recovered during January 2013- May 2015 were studied. Species identification was performed by conventional tests and MALDI-TOF. Antibiotic susceptibility was performed using Phoenix system. Phenotypic screening for KPC was conducted by synergy test, using imipenem- phenyl boronic acid- meropenem disks, and the presence of the blaKPC gene was confirmed by PCR and sequencing. The clonal relatedness among those isolates that displayed unusual susceptibility profiles was evaluated by XbaI-PFGE and Multilocus Sequence Typing. Results:Among 226 KPCKp isolates recovered during the studied period, 197 displayed the classical multidrug-resistant phenotype of ST258, remaining susceptible to colistin and /or tigecycline. However 29 isolates showed susceptibility to two or more families of the following antimicrobial agents: aminoglycosides and/or fluorquinolones and/or trimethoprim-sulfametoxazol. At least 8 different pulsotypes were detected in the PFGE, some included a unique isolate. Also, different STs were observed, some of them corresponding to new allelic profiles (gap-infB-mdh-pgi-phoE-rpoB-tonB): 2-3-91-1-1-1-79; 2-1-91-1-4-4-4. Conclusions:Although multidrug-resistant ST258 constitutes the prevalent KPCKp clone, the emergence of KPCKp displaying a different susceptibility profile is being observed in our hospital, corresponding to sporadic clones that do not display an epidemic behavior.
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Key Words
CarbapenemasesKPCCarbapenem resistance