Science and Technology Production
Biosynthesis of 2,3-dihydroxybenzoic acid in transgenic Catharathus roseus cell cultures overexpressing isochorismate synthase.

Article

Authorship
Muljono, RAB, ; RODRIGUEZ TALOU, JULIAN ; Scheffer, JJC, ; Verpoorte, R.
Date
2002
Publishing House and Editing Place
Elsevier
Magazine
HETEROCYCLES, vol. 56 (pp. 341-352) Elsevier
Summary Information provided by the agent in SIGEVA
The involvement of isochorismate synthase (EC 5.4.99.6) in the biosynthesis of 2,3-dihydroxybenzoic acid (2,3-DHBA), was confirmed by the incorporation of [1-13C]pyruvate in 2,3-DHBA in a transgenic C. roseus cell line constitutively overexpressing the C. roseus plant ics-gene. The label was mainly in the carboxyl group and at C-3 and C-4, which is consistent with isochorismate as intermediate. in the carboxyl group and at C-3 and C-4, which is consistent with isochorismate as intermediate. con... The involvement of isochorismate synthase (EC 5.4.99.6) in the biosynthesis of 2,3-dihydroxybenzoic acid (2,3-DHBA), was confirmed by the incorporation of [1-13C]pyruvate in 2,3-DHBA in a transgenic C. roseus cell line constitutively overexpressing the C. roseus plant ics-gene. The label was mainly in the carboxyl group and at C-3 and C-4, which is consistent with isochorismate as intermediate. in the carboxyl group and at C-3 and C-4, which is consistent with isochorismate as intermediate. constitutively overexpressing the C. roseus plant ics-gene. The label was mainly in the carboxyl group and at C-3 and C-4, which is consistent with isochorismate as intermediate. in the carboxyl group and at C-3 and C-4, which is consistent with isochorismate as intermediate. biosynthesis of 2,3-dihydroxybenzoic acid (2,3-DHBA), was confirmed by the incorporation of [1-13C]pyruvate in 2,3-DHBA in a transgenic C. roseus cell line constitutively overexpressing the C. roseus plant ics-gene. The label was mainly in the carboxyl group and at C-3 and C-4, which is consistent with isochorismate as intermediate. in the carboxyl group and at C-3 and C-4, which is consistent with isochorismate as intermediate. constitutively overexpressing the C. roseus plant ics-gene. The label was mainly in the carboxyl group and at C-3 and C-4, which is consistent with isochorismate as intermediate. in the carboxyl group and at C-3 and C-4, which is consistent with isochorismate as intermediate. The involvement of isochorismate synthase (EC 5.4.99.6) in the biosynthesis of 2,3-dihydroxybenzoic acid (2,3-DHBA), was confirmed by the incorporation of [1-13C]pyruvate in 2,3-DHBA in a transgenic C. roseus cell line constitutively overexpressing the C. roseus plant ics-gene. The label was mainly in the carboxyl group and at C-3 and C-4, which is consistent with isochorismate as intermediate. in the carboxyl group and at C-3 and C-4, which is consistent with isochorismate as intermediate. constitutively overexpressing the C. roseus plant ics-gene. The label was mainly in the carboxyl group and at C-3 and C-4, which is consistent with isochorismate as intermediate. in the carboxyl group and at C-3 and C-4, which is consistent with isochorismate as intermediate. 13C]pyruvate in 2,3-DHBA in a transgenic C. roseus cell line constitutively overexpressing the C. roseus plant ics-gene. The label was mainly in the carboxyl group and at C-3 and C-4, which is consistent with isochorismate as intermediate. in the carboxyl group and at C-3 and C-4, which is consistent with isochorismate as intermediate. C. roseus plant ics-gene. The label was mainly in the carboxyl group and at C-3 and C-4, which is consistent with isochorismate as intermediate.
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