Science and Technology Production
Article
Authorship
WOLSKI, ERIKA ALEJANDRA
;
Murialdo S.E.
;
Gonzalez J.F.
Date
2006
Publishing House and Editing Place
Water Research Comission
Magazine
WATER S.A.,
vol. 32
(pp. 1-5)
Water Research Comission
Summary
Information provided by the agent in
SIGEVA
Pentachlorophenol (PCP) is a toxic compound which is used as a fungicide, bactericide, herbicide and chemical intermediate. Because of its toxicity, there is a need to decontaminate the PCP-laden soils and bioremediation is a very useful alternative, to conventional clean-up methods. The success of this depends on finding strains able to degrade PCP in a changeable environment. The aim of this work was to study the influence of pH of the medium and the effect of inoculum size on pentachlorophen...
Pentachlorophenol (PCP) is a toxic compound which is used as a fungicide, bactericide, herbicide and chemical intermediate. Because of its toxicity, there is a need to decontaminate the PCP-laden soils and bioremediation is a very useful alternative, to conventional clean-up methods. The success of this depends on finding strains able to degrade PCP in a changeable environment. The aim of this work was to study the influence of pH of the medium and the effect of inoculum size on pentachlorophenol degradation by Pseudomonas spp. A kinetic study of PCP degradation was performed to assess such effects. PCP was degraded rapidly at pH values from 6.3 to 8, but the maximum rate of PCP degradation by Pseudomonas sp. was at pH 6.3. In contrast, the kinetic of PCP degradation at pH 5.5 was significantly lower, although PCP was absolutely depleted. These results show the broad range of pH’s for PCP degradation for this strain. PCP was degraded at every inoculum size tested and PCP degradation increased with the increase of the inoculum size, but cultures inoculated with the lowest inoculum showed the highest specific consumption rate. This reveals a lower consumption of PCP per CFU at a high population density. These results are useful to understand the physiological and biochemical properties of Pseudomonas spp. before its optimum use in environmental application and these data will assist help in choosing the right PCP-degrader for a changeable environment.
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