Science and Technology Production
Congress
Authorship
Santiago Manuel, Martín
;
PARIS, RAMIRO
Date
2026
Publishing House and Editing Place
Sociedad Argentina de Fisiología Vegetal
Summary
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SIGEVA
The root cap plays a vital role in protecting the apical meristem and integrating both external and internal signals. Root cap cells originate in the quiescent center and move toward the root tip as the root grows. This dynamic process of differentiation is linked to programmed cell death (PCD). Recent studies suggest that PCD in the root cap is essential for regulating the balance between cell division and cell death, which in turn influences root growth and architecture. In this study, we inv...
The root cap plays a vital role in protecting the apical meristem and integrating both external and internal signals. Root cap cells originate in the quiescent center and move toward the root tip as the root grows. This dynamic process of differentiation is linked to programmed cell death (PCD). Recent studies suggest that PCD in the root cap is essential for regulating the balance between cell division and cell death, which in turn influences root growth and architecture. In this study, we investigated the role of nitric oxide (NO) in the PCD of lateral root cap cells. We utilized propidium iodide (PI) staining to identify cells undergoing PCD and the fluorescent probe DAF-FM 2DA to analyze endogenous NO production in Arabidopsis thaliana roots. Our findings indicate that cells with the highest accumulation of NO are located closer to the quiescent center compared to PI-permeable cells, suggesting that NO may function as an early signal in PCD events. Furthermore, we observed an increased number of PI-permeable cells in roots treated with GSNO, a physiological NO donor, while a reduced number was found in roots treated with cPTIO, a NO scavenger. These results support the hypothesis that NO plays a significant role in regulating PCD in lateral root cap cells during root development. Additionally, studies on mutants with altered transduction pathways and/or levels of NO, as well as of the phytohormone auxin, show varying degrees of PCD in roots. Based on this evidence, we raise new questions regarding how signaling pathways are integrated to ensure proper root growth and development in Arabidopsis thaliana.
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Key Words
RootArabidopsis thalianaMuerte Celular ProgramadaÓxido Nítrico