Science and Technology Production
Congress
Authorship
Date
2023
Publishing House and Editing Place
III Simposio Internacional de Medicina Traslacional
Summary
Information provided by the agent in
SIGEVA
IntroducciónAge related macular degeneration (AMD) affects mainly elder people, impacting negatively and drastically in their quality of life. In underdeveloped countries, many AMD patients have poor access to health care. It is important to analyze inexpensive alternatives to treat them.Amniotic membrane is source of growth factors and has anti-oxidant and anti-angiogenic properties and has reduced cost. ObjetivosTo explore the effect of human amniotic membrane (hAM) on retinal pigment ...
IntroducciónAge related macular degeneration (AMD) affects mainly elder people, impacting negatively and drastically in their quality of life. In underdeveloped countries, many AMD patients have poor access to health care. It is important to analyze inexpensive alternatives to treat them.Amniotic membrane is source of growth factors and has anti-oxidant and anti-angiogenic properties and has reduced cost. ObjetivosTo explore the effect of human amniotic membrane (hAM) on retinal pigment epithelium (RPE) cells exposed to oxidative stress.Material y métodosRPE cells (ARPE-19) were exposed to hydrogen peroxide (H2O2, 0.1-0.4mM) in vitro, to reproduce the oxidative environment in AMD. We analyzed the effect of pre-treatment and post-treatment with hAM available in liquid preparations on wound, propidium iodide/acridine orange (PI/AO) and MTT assays.ResultadosWe observed that after 24hs of exposure to H2O2 (0.2Mm), the RPE cells suffer from cell death, with several nuclei with IP staining. In the wound assay, the complete monolayer start to degenerate and the MTT shown a 60% of survival. Nevertheless, the pre-treatment with 0.2ug/ul of hAM, entirely blocked the effect of H2O2 in the IP/AO and MTT assays. In the wound assay, the cells started to proliferate or migrate in the wound area, nevertheless the cells look thinner than control cells. The post-treatment with hAM showed a 70-80% of survival on MTT and IP/AO assays, but it was lower than that observed with the pre-treatment. In the wound assay, the post-treatment preserves the monolayer, but there were not proliferation or migration in the wound area. ConclusioneshAM could be an inexpensive alternative to reduce the oxidative effects in AMD. We are going to study the anti-angiogenic properties of these preparations of hAM under oxidative conditions and also, the better ways to apply them.
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Key Words
AMNIOTIC MEMBRANEOXIDATIVE STRESSCELL DEATHPROTECTION