Science and Technology Production
Congress
Authorship
Minaberry, Yanina Susana
;
Sagrera, Gabriel
;
García, Mora Renée
;
Gutiérrez, María Laura
;
Svarc, Federico Eugenio
Date
2025
Publishing House and Editing Place
Altex edition
ISSN
2194-0479
Summary
Information provided by the agent in
SIGEVA
Moderate sun exposure has been proven to have various health benefits; however,excessive exposure to ultraviolet (UV) radiation is associated with known adverse effects.Between the three UV ranges (UVA, UVB and UVC), UVA is the dominant component(90–95%). Since UVA penetrates the deepest layers of the skin, it induces the formationof reactive oxygen species (ROS), which contribute to cellular damage. Sunscreens play acrucial role in mitigating these harmful effects.Among the few UVA filte...
Moderate sun exposure has been proven to have various health benefits; however,excessive exposure to ultraviolet (UV) radiation is associated with known adverse effects.Between the three UV ranges (UVA, UVB and UVC), UVA is the dominant component(90–95%). Since UVA penetrates the deepest layers of the skin, it induces the formationof reactive oxygen species (ROS), which contribute to cellular damage. Sunscreens play acrucial role in mitigating these harmful effects.Among the few UVA filters approved in both the United States and Europe, tertbutylmethoxydibenzoylmethane(avobenzone, AVB) is widely used. However, itsphotochemical instability and incompatibility with certain cosmetic ingredients limit itseffectiveness. To address this, the molecule 2-hydroxymethyl-4′-methoxydibenzoylmethane (DBM11) was synthesized as a potential alternative due to its superiorstability under UVA radiation. Initial studies conducted in absolute ethanol under UVirradiation showed negligible decomposition of DBM11, positioning it as a promisingcandidate for sunscreen formulations.In this study, DBM11 synthesis was scaled up to enable its incorporation into a modelemulsion, and its photostability was evaluated under standardized UV irradiationconditions (ISO 24443) in comparison to AVB. Additionally, an essential aspect of theresearch was the evaluation of its safety profile through in vitro phototoxicity testing,following the OECD Test Guideline 432 (3T3 NRU phototoxicity assay), an establishedalternative method to animal testing. This assay provides a reliable prediction ofphototoxic potential using cultured fibroblast cells, reinforcing the commitment toalternative toxicological approaches in cosmetic safety assessment.Results demonstrated that DBM11 exhibits superior photostability in emulsifiedformulations, with slower degradation kinetics compared to AVB, making it a strongcandidate for UVA protection. Furthermore, the in vitro phototoxicity assay classified bothDBM11 and AVB as "probably phototoxic", indicating a comparable safety profile. DBM11represents a promising alternative to AVB, offering enhanced photostability whilesupporting the transition towards animal-free testing strategies in the cosmetic industry.These findings underscore the importance of integrating in vitro methodologies into theevaluation of novel cosmetic ingredients, aligning with the principles of the 3Rs(Replacement, Reduction, Refinement) in toxicology.
Show more
Show less
Key Words
QUIMICA ORGÁNICATOXICIDADDESARROLLOPROTECTOR SOLAR