Science and Technology Production
Congress
Authorship
Baigorri, Ruth Eliana
;
VIANO, ESTEFANIA
;
Hellrriegel, FLORENCIA
;
FONTANARI, CAMILA
;
VAZQUEZ, MATIAS
;
Brugo, María Belen
;
MOTRAN, CLAUDIA CRISTINA
;
RODRIGUEZ GALAN, MC
;
Stempin, C
;
CERBAN, FABIO MARCELO
Date
2024
Publishing House and Editing Place
UNLP
Summary
Information provided by the agent in
SIGEVA
eritoneal cavity is a space that contains fluid with immune cells concentrated 20 times more than blood. Resident peritoneal macrophages (PEMs) consist of two subpopulations with distinct origin, sizes and surface markers. Large Peritoneal Macrophages (LPM) origin in embryonic yolk sac, are CD11b+F4/80+MHC-IIneg and can self-replenish. Small Peritoneal Macrophages are CD11b+MHC-II+F4/80med and derive from blood monocytes. Under homeostasis LPM represents more than 70 percent of total CD11b+ cel...
eritoneal cavity is a space that contains fluid with immune cells concentrated 20 times more than blood. Resident peritoneal macrophages (PEMs) consist of two subpopulations with distinct origin, sizes and surface markers. Large Peritoneal Macrophages (LPM) origin in embryonic yolk sac, are CD11b+F4/80+MHC-IIneg and can self-replenish. Small Peritoneal Macrophages are CD11b+MHC-II+F4/80med and derive from blood monocytes. Under homeostasis LPM represents more than 70 percent of total CD11b+ cells, but during infection or inflammation dampens drastically in a process known as Macrophage Disappearance Reaction. LPMs are essential for T cell priming in T. gondii infection and its pleural analogous control lung L. sigmodontis infection. In T. cruzi infection, we observed a reduction of LPMs during acute phase in susceptible Balb/c mice that did not restore in chronic phase. Instead, this population was replaced by ?Converting macrophages?, which do not acquire LPM maturation characteristics. We hypothesized that restoring LPM in the peritoneal cavity could control parasite replication and enhance T cell response. To investigate how the replenishment of LPMs impacts the response to T. cruzi infection, we transferred PEMs to infected mice early in the acute phase by i.p. injection. Four days later, blood samples, peritoneal lavages and spleens were collected. Infected animals without PEMs transfer and non-infected animals were analyzed in parallel. We found that mice transferred with PEMs showed less parasitemia than non-transferred infected animals (p<0.05). Both spleen and peritoneal T cells showed the same CD4 activation but CD8 T cells exhibited higher PD-1 positive cells than non-transferred counterparts (p<0.05). Spleen CD8 T cells showed more frequencies of effector (CD44+CD62-Lneg) cells (p<0.01). These findings suggest that LPM restoration may be a promising strategy to enhance immune response and control parasitic replication during T. cruzi infection.
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Key Words
MACROPHAGEST. CRUZIIMMUNE RESPONSE