Science and Technology Production
Congress
Authorship
JACOB, JULIETA
;
Castro Pérez, Santiago
;
Reta, Pablo
;
Vieyra, Cynthia
;
Fader Kaiser, Claudio M.
Date
2024
Publishing House and Editing Place
BIOCELL
Summary
Information provided by the agent in
SIGEVA
The erythroid precursors go through physiological changes during erythropoiesis that are important for the maturation of the red blood cells. These changes involve the remodelling of the membrane, the decrease in cell volume, the production of hemoglobin and organelle clearance, such as mitochondria and ribosomes, after enucleation. Autophagy is a cellular process related to the engulfment of cytosolic macromolecules and whole organelles into double membrane vesicles called autophagosomes, whic...
The erythroid precursors go through physiological changes during erythropoiesis that are important for the maturation of the red blood cells. These changes involve the remodelling of the membrane, the decrease in cell volume, the production of hemoglobin and organelle clearance, such as mitochondria and ribosomes, after enucleation. Autophagy is a cellular process related to the engulfment of cytosolic macromolecules and whole organelles into double membrane vesicles called autophagosomes, which then fuse with lysosomes for the cargo degradation. During erythropoiesis, mitophagy and ribophagy play a crucial role in enabling the proper maturation of red blood cells. A transmembrane receptor known as LRP1 (low density lipoprotein receptor-related protein 1) engages in a variety of cell processes, including proliferation, differentiation, metabolism, apoptosis, autophagy, and the degradation of the hemin-hemopexin complex. To address the role of LRP1 in autophagy stimulation, we generated two different LRP1 knockdown hematopoietic cell lines (K562 and UT7), using the lentivirus approach. Both cell lines were derived from patients with chronic myeloid leukemia and are widely used as models for erythroid maturation and differentiation. We demonstrate that alpha-2 macroglobulin (LRP1 ligand) triggers the autophagic pathway in a LRP1 dependent manner using confocal immunofluorescence, electron microscopy, and western blot. The study of autophagy in relation to the role of the LRP1 receptor during differentiation and erythropoietic maturation is crucial for the creation of potential treatments for several hematopathologies, including anemia and leukemia.
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Key Words
ErythropoiesisLRP1LeukemiaAutophagy