Science and Technology Production
Congress
Authorship
Rolando, A.L.
;
POMBO, MARINA ALEJANDRA
;
Rosli H.G.
Date
2025
Publishing House and Editing Place
XXXV Reunión de la Sociedad Argentina de Fisiología Vegetal
Summary
Information provided by the agent in
SIGEVA
To defend themselves against pathogens plants have evolved two interconnected immune responses. Pattern-triggered immunity (PTI) is activated by the perception of microbe-associated molecular patterns (MAMPs), while effector-triggered immunity (ETI) depends on the recognition of effectors. Usually ETI culminates in a hypersensitive response (HR) with programmed cell death (PCD). In some tomato lines, resistance to Pseudomonas syringae pv. tomato (Pst) DC3000 relies on the recognition of AvrPto ...
To defend themselves against pathogens plants have evolved two interconnected immune responses. Pattern-triggered immunity (PTI) is activated by the perception of microbe-associated molecular patterns (MAMPs), while effector-triggered immunity (ETI) depends on the recognition of effectors. Usually ETI culminates in a hypersensitive response (HR) with programmed cell death (PCD). In some tomato lines, resistance to Pseudomonas syringae pv. tomato (Pst) DC3000 relies on the recognition of AvrPto and AvrPtoB effectors by Pto and Prf. Previously, we demonstrated that NbATG1-silenced N. benthamiana plants exhibit delayed AvrPto/Pto-mediated PCD and disease-associaded symptoms. To further assess NbATG1 participation in ETI, we now infiltrated N. benthamiana silenced plants with Pst DC3000 to evaluate if PCD due to the recongition of another bacterial effector (HopQ1-1 through Roq1) is affected. We observed a delayed PCD response in NbATG1 silenced plants compared with Ec1 control. We had previously shown a PCD delay using two different pairs of resistance proteins and non-bacterial elicitors. To extend this analysis, we now tested the RPP13/ATR13EmcoΔ41 pair from the oomycete Hyaloperonospora parasitica and Arabidopsis, respectively. In this case PCD was delayed in NbATG1-silenced plants. Similar experiments were performed using Bax, a murine proapoptotic protein that triggers cell death in plants, and no differences were found between NbATG1- and Ec1- silenced plants. Taken together these results indicate that NbATG1 is required for the development of PCD associated to two bacterial effectors and to non-bacterial elicitors.
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Key Words
Nicotiana benthamianaPathogenAutophagyImmunityProtein kinase