Science and Technology Production
Book Chapter
Authorship
Arturo Redondo-López
;
Nahuel González Schain
;
Mariano Perales
;
Daniel Conde
Date
2025
Publishing House and Editing Place
Springer
Book
CRISPR-CAS Methods Vol.3
(pp. 227-243)
Springer
Springer
ISBN
978-1-0716-4358-7
Summary
Information provided by the agent in
SIGEVA
The generation ofknock-out mutant lines to null the function of one or multiple genes is nowadaysthe requested quality standard to unequivocally resolve novel regulatorygenetic pathways. CRISPR/Cas system has become the most straightforward tool inwoody perennials research for this purpose. Moreover, the worldwide tendencyregarding the regulation for the commercialization of products derived fromgenome-edited plants is evolving so that these plants are not considered GMOswhen foreign DNA is rem...
The generation ofknock-out mutant lines to null the function of one or multiple genes is nowadaysthe requested quality standard to unequivocally resolve novel regulatorygenetic pathways. CRISPR/Cas system has become the most straightforward tool inwoody perennials research for this purpose. Moreover, the worldwide tendencyregarding the regulation for the commercialization of products derived fromgenome-edited plants is evolving so that these plants are not considered GMOswhen foreign DNA is removed. Considering this, together with the potential oftargeted gene editing to improve any desired trait in agroforestry, such asfruit, wood, or biomass production, results in more and more researchersinterested in adopting the CRISPR/Cas system for their investigations in woodyplants. Here we describe the different methodological aspects that a researchermust consider and perform a comparative analysis between the options tested inthe last years for this purpose in woody perennials, offering a valuableresource for a step-by-step decision-making process. Eliminating the transgenicscarfs of targeted genome editing, i.e., the Cas DNA sequence and othertransgenic sequences within the genome after plant regeneration, is particularlydifficult for perennial plants, where segregation by sexual seed productiontakes an extremely long period. In this chapter, we also summarize thepotential strategies to face the challenge of applying next-generation plantbreeding to create commercial genotypes with high ecological and economic valuein trees.
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Key Words
next-generation plant breedingwoody perennialsCRISPR/Castargeted genome editing