Science and Technology Production
Congress
Authorship
Date
2023
Publishing House and Editing Place
Fundación Hongos de Argentina para la Sustentatbilidad
Summary
Information provided by the agent in
SIGEVA
Thecaphora frezii, the causal agent of peanut smut, overwinters in soil and crop residues as teliospores. When peanut pegs penetrate the soil, the telial dormancy is disrupted and the spores germinate, initiating the infection of the gynophore aiming to replace the cells with teliospores as part of the fungus life cycle. The objective of this work was to perform a microscopic analysis at the beginning of the interaction between the gynophore and T. frezii, with emphasis on defense-related compo...
Thecaphora frezii, the causal agent of peanut smut, overwinters in soil and crop residues as teliospores. When peanut pegs penetrate the soil, the telial dormancy is disrupted and the spores germinate, initiating the infection of the gynophore aiming to replace the cells with teliospores as part of the fungus life cycle. The objective of this work was to perform a microscopic analysis at the beginning of the interaction between the gynophore and T. frezii, with emphasis on defense-related components such as lignin, suberin and tannins. Arachis hypogaea L. seeds from Granoleico (susceptible) and Ascasubi (highly tolerant) varieties were planted in pots and maintained in the greenhouse. Each pot was inoculated with T. frezii spores at the blooming stage. After 72 h of gynophore penetration into the soil, samples of 1 cm were obtained to perform microtome or freehand cross-sections. Some slides were examined by epifluorescence microscopy, while others were stained with Toluidine Blue or WGA-Alexa 488 and observed by confocal microscopy. The morphological features of the developing ovule and embryo were similar in the two varieties inoculated with T. frezii. In addition, idioblasts containing tannins adjacent to the xylem, suberin, and lignin from the fibers associated to the phloem were observed. The presence of T. frezii structures were only observed in samples from Granoleico. The data obtained with the techniques used, suggest that there are no morphological or anatomical differences between the gynophores from Granoleico and Ascasubi varieties, after 72 h of penetrating the soil inoculated with T. frezii spores.
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Key Words
INTERACTIONARACHIS HYPOGAEAPEANUT SMUT