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Medicina - NEUROACTIVE STEROIDS ESTROGEN AND PROGESTERONE INDUCE NEUROPROTECTION BY DELAYING SIGNS OF DEPRESSION AND MOTOR IMPAIRMENT IN ORCHIECTOMIZED MALE HEMIPARKINSONIAN RATS
Congress
Authorship:
Natalia González ; Trinidad Parra ; Ignacio Moreno ; Esther Vargas ; Emiliana Mashad ; Ricardo CabreraDate:
2023Publishing House and Editing Place:
MedicinaSummary *
Parkinson’s disease (PD) is a neurodegenerative pathology. This disease is characterized by an irreversible loss of dopaminergic neurons in the substantia nigra. PD is more prevalent in men. Nigrostriatal degeneration is studied with experimental models in rodents by unilateral intrastriatal injection of the neurotoxic 6-hydroxydopamine (6-OHDA), which induces dopaminergic neurodegeneration and hemiparkinsonism in rats. The objective was to evaluate the neuroprotection induced by progesterone (P) and estrogen (E) on the motor alterations associated with neurodegeneration in orchidectomized male hemiparkinsonian rats. Adult male Sprague-Dawley rats were used, and neurodegeneration was induced by microinjection of 6-OHDA into the striatum. The experimental groups were: uncastrated control (C), hemiparkinsonian (HP), E-treated hemiparkinsonian (HPE), and P-treated hemiparkinsonian (HPP). Castrated control (CO), castrated hemiparkinsonian (HPO), castrated hemiparkinsonian treated with E (HPEO), and castrated hemiparkinsonian treated with P (HPPO). The animals were evaluated in two motor variables: forced swimming (PNF) and Raturn. Data were expressed as mean+SEM and analyzed by ANOVA 2 and Bonferroni post hoc. We observed a significant decrease in PNF in the swimming time of the C’s with respect to CO (p<0.0001). No significant differences were observed between Cs with respect to HPE and HPP. HP animals showed a significant decrease in swimming time with respect to C (p<0.0001). A significant increase in this variable was observed in the HPPO and HPEO groups with respect to HP (p<0.0001). In the raturn test, the HP group presented a significant increase in rotational activity with respect to the C group (p<.0001). The HPP and HPE groups presented a significant decrease with respect to HP, bringing them to values of C. In the orchidectomized animals we observed a significant decrease in contralateral gyrations in HPPO and HPEO with respect to HP (p<0.0001). We conclude that both E and P have a neuroprotective effect enhanced by the decrease in testosterone induced by orchidectomy. Information provided by the agent in SIGEVAKey Words
HEMIPARKINSONIAN RATSDEPRESSIONORCHIEDECTOMYNEUROACTIVE STEROIDS