Science and Technology Production
Book Chapter
Authorship
Date
2023
Publishing House and Editing Place
Frontiers
Book
Helicobacter pylori infection: Pathogenesis, antibiotic resistance, advances and therapy, new treatment strategies
(pp. 70-79)
Frontiers
Frontiers
ISBN
978-2-83251-123-7
Summary
Information provided by the agent in
SIGEVA
Helicobacter pylori infection is widespread worldwide, with more than a halfof the world population infected. H. pylori antibiotic-resistant strains and noncomplianceto therapy are the major causes of H. pylori eradication failure.The search for new therapies based on plant extracts is a scientific interestfield. The present study was conducted to evaluate the effect in vitro of extravirgin olive oil (EVOO), hydroxytyrosol (HT), and oleuropein (Olp) against twoH. pylori strains and the effect i...
Helicobacter pylori infection is widespread worldwide, with more than a halfof the world population infected. H. pylori antibiotic-resistant strains and noncomplianceto therapy are the major causes of H. pylori eradication failure.The search for new therapies based on plant extracts is a scientific interestfield. The present study was conducted to evaluate the effect in vitro of extravirgin olive oil (EVOO), hydroxytyrosol (HT), and oleuropein (Olp) against twoH. pylori strains and the effect in vivo of the oral administration of EVOOon the gastric mucosa of BALB/c mice infected with this microorganism.The broth microdilution method assayed the antibacterial in vitro activityof EVOO, HT, and Olp against H. pylori strains. For in vivo studies, maleBALB/c mice were infected orally with an H. pylori suspension every 72 h.Four groups were used: (1) Control, (2) H. pylori-infected (HP), (3) EVOO, and(4) HP + EVOO. Mice were sacrificed at 7, 15, and 30 days. The stomachswere removed and observed under a microscope. Scoring of the degree oferosion was determined. Samples were processed by histological techniquesfor light microscopy. Macroscopic analysis showed that the presence of smallerosions increased, both in number and size, in the infected group. Animalsinfected and treated with EVOO exhibited the presence of fewer erosions,which decreased in number as the treatment progressed. The mucosa ofthe control and EVOO groups showed normal histological characteristicsat the three times studied. The mucosa of animals infected with H. pylorishowed disruptions of the lining epithelium, damage to gastric glands, andvasodilation. The mucosa of animals infected with H. pylori and treated withEVOO showed morphological characteristics similar to those of normal andEVOO mucosa. For the first time, the current study showed the effect in vitroand in vivo of EVOO and combined administration of HT and Olp against H. pylori using an animal model. Future studies are needed to establish themechanism of EVOO?s action at the gastric mucosa level to propose thisproduct as a natural antimicrobial agent for the treatment of gastric H. pyloriinfections.
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Key Words
GASTRIC ULCERHELICOBACTER PYLORIVIRGIN OLIVE OILANTIOXIDANT