Science and Technology Production
Reproduction in Domestic Animals - Anti-Müllerian hormone under short and long photoperiods in female cats
Congress
Authorship:
Lapuente Camila ; Lantermino, Augusto ; Moabro Daniela ; Eaton Daiana ; Silva. A ; Arbizu. J ; Blanco Paula ; Gobello CristinaDate:
2023Publishing House and Editing Place:
Jean-François BRUYASSummary *
Anti-Müllerian hormone (AMH) is a glycoprotein belonging to the transforming growth factors (TGF-β). In females, AMH is secreted by granulosa cells of small follicles in the ovary. In most seasonal species AMH varies throughout the different reproductive seasons. Although the domestic cat has been classified as a reproductively seasonal species, nothing is known about the effect of photoperiod on AMH serum concentrations in queens. The objective of this study was to describe and compare AMH serum concentrations under a short (SP) vs a long (LP) artificial photoperiod.Five, 2.5 years female cats were housed free in a windowless room with LED lamps under a short (SP, 6:18 L/D) and a long photoperiod (LP, 16:8 L/D) of 3 months (IACUC V269).A blood sample was collected from the jugular vein at the end of the 2nd and 3rd month of both SP and LP at 6 h am. Serum was stored frozen at -70°C until AMH analysis (Elecsys, Cobas, Roche Diagnostics International Ltd., Switzerland). The sensibility and the intraassay CV of the kit were 0.01 n/ml and < 5%, respectively.Normality of AMH serum values was confirmed by Shapiro-Wilk test. AMH concentrations were analyzed by paired and unpaired Student t tests. The level of significance was set at P<0.05.AMH serum concentrations had a tendency to decrease in the 2nd sample of the SP (P= 0.1), but it did not vary during the LP (P>0.1). Although serum AMH concentrations were 32% lower in LP than in SP (2.76±0.54 vs. 3.66±0.49 ng/m; P= 0.2) significancy was not attained with this number of animals. This lower AMH levels could be explained by the presence of large antral ovarian follicles during this stimulating photoperiod. Information provided by the agent in SIGEVAKey Words
CATREPRODUCTIONANTI-MULLERIAN HORMONEPHOTOPERIOD