Science and Technology Production
Congress
Authorship
Farinati, Alicia Esther
;
Fernández Núñez, Agustín
;
Rollet, Raquel
;
Quinteros, Mirta
;
CONFORTE, VALERIA PAOLA
;
Cassará, María Luz
;
Luna, Paula
Date
2022
Publishing House and Editing Place
ASM
Summary
Information provided by the agent in
SIGEVA
Background. Xylitol (Xy) is a polyalcohol that is used in the food industry toreplace sucrose. It has proven antimicrobial activity on various microorganisms ofclinical interest. Our aim was to study the activity of Xy on the formation ofbiofilms (BFs) in infectious skin diseases: atopic dermatitis (AD) and severe acne(SA). Methods. For the study of AD lesions, 34 patients aged 3 to 17 years wereincluded. Samples of active lesions were obtained with a swab moistened withsterile physiological so...
Background. Xylitol (Xy) is a polyalcohol that is used in the food industry toreplace sucrose. It has proven antimicrobial activity on various microorganisms ofclinical interest. Our aim was to study the activity of Xy on the formation ofbiofilms (BFs) in infectious skin diseases: atopic dermatitis (AD) and severe acne(SA). Methods. For the study of AD lesions, 34 patients aged 3 to 17 years wereincluded. Samples of active lesions were obtained with a swab moistened withsterile physiological solution. Likewise, 10 patients with SA lesions were included.The samples were taken after antisepsis of the lesion area with special strips toextract the hair follicle where the bacteria are housed. The DA and SA samples werepreserved for the isolation of bacteria present in them and the study of the effect ofXy on the formation of their BFs. The adhesion and dispersion of the BFs in thepresence of Xy 7% were analyzed by previous microscopic evaluation of crystalviolet staining, assigning a score to each sample. This score was based on the typeof development of BF with a scale: 0 indicator of absence, 5 the maximum valueobtained, depending on the degrees of development and number of bacteria in them.We carry out complementary studies with confocal microscopy. Results. BF-forming S. aureus was isolated from the AD lesions analyzed, 26 cases wereevaluated for adherence and 27 for dispersion. For adherence, the mean score wassignificantly higher after the intervention with Xy (3.67 ± 1.21 vs 2.35 ± 1.13,respectively; p<0.05, Wilcoxon test); 65.4% of the samples showed a significantreduction in the score after the intervention, while the remaining 34.6% remainedthe same or had a worse score compared to baseline. Dispersion of the BF wasverified in 70.4% of the samples with the addition of Xy (of at least 1 point). In SA,the predominant BF-forming bacterium was Cutibacterium acnes (CA), verifyingthe reducing activity of Xy in adherence and the facilitation of BF dispersion withrespect to the corresponding controls. Conclusions. Xy has reliable antimicrobialactivity against SA BFs in AD and in SA cases, against CA. These results supportits application for the treatment of these infectious pathologies, contributing to thereduction of the use of antibiotics and corticosteroids.
Show more
Show less
Key Words
BIOPELÍCULASACNÉDERMATITIS ATÓPICAXILITOL