Science and Technology Production
BIOCELL - IN VIVO BIOCOMPATIBILITY OF HYDROGELS BASED ON POLY-N-ISOPROPYLACRYLAMIDE (PNIPAM)
Congress
Authorship:
Arri, Jimena ; Capella, Virginia ; Babini, Sandra ; Liaudat, Ana Cecilia ; BOSCH, PABLO ; Rivarola, Claudia R. ; Rodriguez, NancyDate:
2021Publishing House and Editing Place:
Tech Science PressSummary *
Over the last decades, biomaterials have been developed with great potential to be applied as scaffolds for cell growth, expanding the alternatives to organ and tissue transplants, within the field of tissue engineering. These scaffolds, in addition to allowing a correct in vitro development of the tissue of interest, must be accepted by the host avoiding any type of immunological rejection and must also be biostable in the time required for the desired tissue regeneration. Instead, its use in vivo introduces a relevant variable that must be studied: the interaction of the biomaterial with the cells belonging to the host´s immune system. Hydrogels, especially those based on poly-N-isopropylacrylamide (PNIPAM), have been among the most studied in our group due to their similarity with the extracellular matrix (EMC). Based on this background, the aim of this study was to analyze the biocompatibility and immunological acceptance with three-dimensional (3D) hydrogels based on PNIPAM and its co-polymer, PNIPAM-co-3% APTA (3-acrylamidopropyl chloride) trimethyl- ammonium), in murine models of the Wistar strain. For this purpose, hydrogel discs were implanted in subcutaneous pockets, after sterilizing the materials. In the controls, the same procedure was performed without implanting the hydrogel. The healing process was monitored for 5 days, and the materials were recovered after 3 months. After this stage, the individuals were sacrificed, and blood samples were taken for hematological and biochemical analysis. No alterations in the healing process were observed regarding to controls in both treatments. Also, there were neither significant changes in the leukocyte formula, compatible with inflammatory processes, nor in the blood biochemistry that can be suggestive of kidney and liver dysfunction. Hence, these preliminary studies indicate that PNIPAM-based hydrogels do not generate immune rejection or significant alterations in the hematology and blood biochemistry of Wistar rats in a chronic period of 3 months. Information provided by the agent in SIGEVAKey Words
HYDROGELBIOMATERIALSCELL THERAPYBIOCOMPATIBILITY