Science and Technology Production

Placenta - ORAL INFECTIONS AND PREGNANCY OUTCOME: OUTER MEMBRANE VESICLES FROM PORPHYROMONAS GINGIVALIS IMPAIR TROPHOBLAST CELL FUNCTION

Congreso

Authorship:

Lara, Brenda ; MERECH, FÁTIMA ISABEL ; Calo, Guillermina ; Gliosca, Laura ; Vota, Daiana ; Ramhorst, Rosanna ; Pérez Leirós, Claudia ; Hauk, Vanesa

Date:

2022

Publishing House and Editing Place:

Elsevier

ISSN:

0143-4004

Summary *

Objectives: Adverse pregnancy outcomes and deficient placentation areassociated with periodontal disease. Porphyromonas gingivalis (Pg) is apathogen characteristic of periodontal disease. Pg release outer membranevesicles (Pg-OMV) that increase their pathogenicity and survival. Our aimwas to evaluate the effect of Pg-OMV on trophoblast cell function andmetabolism.Methods: Pg-OMV were isolated by ultracentrifugation and characterizedusing dynamic light scattering and transmission electron microscopy. HTR-8 human first-trimester trophoblastic cell line was treated with 0.1-10 mg/ml Pg-OMV for 2-24h depending on the assay. Cell migrationwas analyzedby wound healing assays and cell invasiveness in Matrigel-coveredtranswells. Glucose and fatty acid uptake were evaluated by flow cytometryusing the fluorescent analog 2-NBDG and FLC12 Bodipy probe,respectively. Cellular apoptosis was assessed by flow cytometry usingannexin V/propidium iodide. Indoleamine 2,3-dioxygenase-1 (IDO) andhigh-mobility group box 1 (HMGB1) mRNA expression were measured byRT-qPCR.Results: We observed that Pg-OMV enter the trophoblast cells in a timeand concentration-dependent manner. Treatment of trophoblast cells withPg-OMV reduced migration at 18h (p<0.05) and invasiveness capacitywithout altering trophoblast apoptosis rate. We also found that Pg-OMVdiminished glucose uptake without modifying fatty acid uptake. Finally,Pg-OMV increased the expression of HMGB1 mRNA among other proinflammatorymediators, whereas they decreased IDO expression (p<0.05).Conclusion: Our results indicate that Pg-OMV impairs trophoblast cellfunction, reduces glucose uptake, and promotes a proinflammatory profile.These findings might contribute to elucidating the mechanisms underlyingthe impact of periodontal infection during placentation. Information provided by the agent in SIGEVA

Key Words

TROPHOBLASTPORPHYROMONAS GINGIVALISOUTER MEMBRANE VESICLES