Producción CyT
256th ACS meeting - Association between a SNP and cytochrome P450-mediated herbicide resistance in Lolium spp. populations
Congreso
Fecha:
2018Editorial y Lugar de Edición:
ACSResumen *
Multiple resistance to ACCase- and AHAS/ALS-inhibiting herbicides in Lolium spp. populations increases weed management costs in winter cereal production systems from Argentina. In most cases, cytochrome P450-mediated herbicide metabolism seems to be involved. The objective of this work was to develop markers associated with herbicide metabolism mediated by cytochrome P450. Genomic DNA was isolated from herbicide-resistant and susceptible plants obtained from a Lolium spp. population with multiple resistance (pinoxaden, iodosulfuron-methyl/mesosulfuron-methyl and pyroxsulam) mediated by cytochrome P450 monooxygenases. Five primers were designed from consensus regions of multiple cytochrome P450 sequences. Different levels of DNA polymorphism were detected combining the primers into pairs.A SNP was presumably related to the multiple herbicide resistance, where G and A alleles would be related to herbicide resistance and susceptibility, respectively. Tetra primer ARMS PCR method was developed for genotyping accessions obtained from 20 Lolium spp. populations (13 populations with multiple herbicide resistance to AHAS/ALS and ACCase inhibitors and 7 susceptible populations) from Argentina. The G or A allele-specific amplicon (140 and 225 bp, respectively) was detected in 90% of the cases. G allele was significantly associated to multiple herbicide resistance (p<0.01) and it was detected in nine populations which showed resistance to pinoxaden, iodosulfuron-methyl/mesosulfuron-methyl, pyroxsulam and/or sulfometuron-methyl/chlorimuron-ethyl. In these populations, herbicide detoxification was corroborated with cytochrome P450 inhibitors (malathion, piperonyl butoxide and 1-aminobenzotriazole). The A allele in homozygous state was detected in all accessions from susceptible populations. Future work will widen the number of populations studied. Información suministrada por el agente en SIGEVAPalabras Clave
RYEGRASSRESISTANCEHERBICIDES