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STIGLIANO, JOSE NICOLAS

INTERNA DOCTORAL TEMAS ESTRATEGICOS

SPECIALITY:

Regulacion de la expresion genica

Scientific discipline:

Bioquímica y Biología Molecular

Topic:

Estudio de los patrones de splicing alternativo y expresión génica en órganos de ratones modelo para la atrofia muscular espinal (AME) humana sometidos a distintos tratamientos terapéuticos.

Workplace

INSTITUTO DE FISIOLOGIA, BIOLOGIA MOLECULAR Y NEUROCIENCIAS (IFIBYNE, CONICET-UBA) Depends on
Address:
INTENDENTE GUIRALDES 2160, piso 2, C1428EGA - Capital Federal - Argentina

Contact:

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S&T Expertise*

1. SEP-DEC 2017. Investigated how specific histone post translational modifications and ATP dependent chromatin remodeling enzymes interact in the regulation of global chromatin organization. Experience: transformations, yeast cell culturing, haploid mating, sporulation and tetrad dissection, PCR, spectrophotometry, micrococcal nuclease digestion, agarose gel electrophoresis, mononucleosomal DNA isolation, DNA fragment end repair, adaptor ligations, barcode integration, serial dilutions, Illumina deep sequencing analysis and Qubit Fluorometric Quantitation.2. JUN-AUG 2017 IFIBYNE. Assisted with experiments relating to the epigenetic regulation of alternative splicing levels in SMA affected patient samples and treatment using histone modifiers and ASO. Experience: human cell culturing, transfection, serial dilutions, RNA isolation, retrotranscription, PCR, agarose gel electrophoresis, radioactive PCR, western blots, qPCR. 3. FEB-MAR 2017 UoD. Constructed Synthetic Biology device to combat bacterial resistance by using RNAi to silence E.coli chloramphenicol resistance genes. *Information provided by the agent in SIGEVA

Lines of Investigation

Chromatin organization, Epigenetic regulation of alternative splicing levels, Use of BioBrick (iGem). CIENCIAS NATURALES Y EXACTAS - Ciencias Biológicas - Bioquímica y Biología Molecular (ídem 3.1.10)

Technological Capacities

  • 6. Ciencias biológicas
    • 2. Biología / Biotecnología
      • 2.2. Biología celular y molecular

Key Words

EpigeneticaEpigeneticsBiologia SinteticaBiologia MolecularSynthetic BiologyMolecular Biology

Education

2014 - 2018

Licenciado (Bachelor of Science (Honours) Molecular Biology)

UNIVERSITY OF DUNDEE


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