1. CONICET
  2. CONICET Community
  3. PABLO MARIANO FERNANDEZ
CONICET Community
FERNANDEZ, PABLO MARIANO

Adjunctive researcher

Speciality
Immnulogia Reproductiva
Scientific discipline
Medical Sciences - Biochemistry and Molecular Biology
Topic
Búsqueda de marcadores específicos de un embarazo exitoso en células T del sistema inmune de mujeres con fallas reproductivas reproductivas.
Workplace
INSTITUTO DE INMUNOLOGIA, GENETICA Y METABOLISMO (INIGEM, CONICET-UBA)
Dependencies
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Address:
AV. CORDOBA 2351, piso 4, C1120AAR - Capital Federal - Argentina
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Summary Information provided by the agent in SIGEVA
Doctor en Bioquímica, Biologia Molecular. Trabajé para el desarrollo de productos biotecnológicos para la prevención y tratamiento de enfermedades en humanos, y actualmente estudio la expresión de receptores nucleares huérfanos en células del sistema inmune en el contexto del embarazo. Technical Skills: Expression (E.coli, HEK293), purification and refolding of proteins. Assay. PAGE. Biophysical and structural characterization (Biacore, ultracentrifugation, DLS, CD, Mass Spectrometry). Crystal ... Doctor en Bioquímica, Biologia Molecular. Trabajé para el desarrollo de productos biotecnológicos para la prevención y tratamiento de enfermedades en humanos, y actualmente estudio la expresión de receptores nucleares huérfanos en células del sistema inmune en el contexto del embarazo. Technical Skills: Expression (E.coli, HEK293), purification and refolding of proteins. Assay. PAGE. Biophysical and structural characterization (Biacore, ultracentrifugation, DLS, CD, Mass Spectrometry). Crystal growth. Functional characterization: activity, interactions, immunology. ELISA. Determination of kinetic parameters of protein kinases. Screening inhibitors. Characterization of antibodies. Cloning and expression of genes. Preparation of DNA and RNA samples. Dot, Northern, Southern, Western blots, labeling and hybridization (membranes and tissu). EMSA. PCR. Sequencing. Site-directed mutagenesis and gene knockout. Screening libraries. Two-hybrid. Optical microscopy. Flow cytometry. Cell Culture: eukaryotic cells, bacteria, and parasites (P2 and P3). Transfection. Sub-cellular fractionation.
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Lines of Investigation

Inmunogenética y Mecanismos Moleculares de Activación/Inhibición

Natural and exact sciences

  • Biological sciences
  • Biochemistry and molecular biology (same as 3.1.10)
Technological Capacities

6 - Biological sciences

6.1 - Medicine, Human Health

  • 6.1.1 - Biostatistics, Epidemiology
  • 6.1.2 - Clinical Research, Trials
  • 6.1.5 - Diagnostics, Diagnosis
  • 6.1.10 - Medical Research
  • 6.1.16 - Virus, Virology/Antibiotics/Bacteriology
  • 6.1.23 - Human vaccines

6.2 - Biology/Biotechnology

  • 6.2.1 - Biochemistry/Biophysics
  • 6.2.2 - Cellular and Molecular Biology
  • 6.2.3 - Genetic Engineering
  • 6.2.4 - In vitro Testing, Trials
  • 6.2.5 - Microbiology
  • 6.2.6 - Molecular design
  • 6.2.11 - Protein Engineering

6.3 - Genome Research

  • 6.3.1 - Bioinformatics
  • 6.3.2 - Gene Expression, Proteom Research
  • 6.3.3 - Population genetics
Key Words
Expresión de Factores de TranscripciónInmunologiaTolerancia InmunológicaImmune ToleranceImmunologyTranscription Factor Expression
Education

1998 - 2000

Doctor en Bioquimica

UNIVERSIDAD NACIONAL DE SAN LUIS

1994 - 1996

Master en Biologia Molecular e Ingeneria Genetica

ESCUELA SUPERIOR DE ECONOMIA Y ADMINISTRACION DE EMPRESAS

1987 - 1993

Licenciado en Bioquimica

UNIVERSIDAD NACIONAL DE SAN LUIS

1987 - 1993

Profesor de Enseñanza Media y Superior en Quimica

UNIVERSIDAD NACIONAL DE SAN LUIS

HR Training
Director of:
MIRA, KAREN
Scholars

MIRA, Karen Scholars

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