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  3. EZEQUIEL NAZER
CONICET Community
NAZER, EZEQUIEL

Assistant researcher

Speciality
Biología Molecular y Celular
Scientific discipline
Biochemistry and Molecular Biology - Medical Sciences
Topic
Estudio funcional y molecular de la proteína argonauta 1 (AGO1) en la activación de enhancers dependiente de estrógenos
Workplace
INSTITUTO DE FISIOLOGIA, BIOLOGIA MOLECULAR Y NEUROCIENCIAS (IFIBYNE, CONICET-UBA)
Dependencies
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Address:
INTENDENTE GUIRALDES 2160, piso 2, C1428EGA - Capital Federal - Argentina
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Summary Information provided by the agent in SIGEVA
Breast cancer is the leading cause of cancer-related death in women. Positive outcomes can be attributed to success with targeting hormone receptors in hormone receptor-positive disease, however, a significant fraction develops resistance and progresses to metastatic disease. At the molecular level, perturbations of cell type-specific enhancers and their transcriptional programs play key roles in tumorigenesis. We have recently shown a non-canonical role for Argonaute1 (AGO1) as coactivator of ... Breast cancer is the leading cause of cancer-related death in women. Positive outcomes can be attributed to success with targeting hormone receptors in hormone receptor-positive disease, however, a significant fraction develops resistance and progresses to metastatic disease. At the molecular level, perturbations of cell type-specific enhancers and their transcriptional programs play key roles in tumorigenesis. We have recently shown a non-canonical role for Argonaute1 (AGO1) as coactivator of estrogen-induced enhancers. ChIP-seq analysis showed that AGO1 colocalizes with estrogen-responsive enhancers in MCF7 cells (ER+ breast cancer cell line) in an estrogen-dependent manner. Interestingly, coimmunoprecipitation assays showed that AGO1 interacts with ER upon estradiol treatment. AGO1 modulates the recruitment of ER onto induced-enhancers, activates enhancer transcription, enhances induced enhancer-promoter interactions and activates target promoters in response to E2. Overall, these results suggest that AGO1 plays a critical role during the activation estrogen-induced enhancers.
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Lines of Investigation

Regulación de la expresión génica

Natural and exact sciences

  • Biological sciences
  • Biochemistry and molecular biology (same as 3.1.10)
Technological Capacities

6 - Biological sciences

6.2 - Biology/Biotechnology

  • 6.2.2 - Cellular and Molecular Biology

6.3 - Genome Research

  • 6.3.1 - Bioinformatics
  • 6.3.2 - Gene Expression, Proteom Research
Key Words
ChromatinTranscriptionEnhancers CromatinaTranscripción
Education

2006 - 2011

Doctor en Biología Molecular y Biotecnología

UNIVERSIDAD NACIONAL DE SAN MARTIN

1999 - 2004

Licenciado en Ciencias Biológicas

UNIVERSIDAD DE BUENOS AIRES

HR Training
Co-director of:
WU, GRACE
Scholars

WU, Grace Scholars

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